Application Notes

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Advanced biological models and credible reference standards enable greater specificity and functionality to the researcher’s toolkit. Browse our growing collection of application notes for access to in-depth data on the development and validation of these products and to explore proof-of-concept studies focused on their various applications.
Blue and green mouse embryo fibroblast. Application note

3T3-L1 Cells Lose Contact Inhibition and Adipogenic Potential When Maintained in Culture at Confluence

This study will determine if 3T3-L1 adipocyte differentiation is dependent on contact inhibition, and will test the OP9 cell line as a possible substitute for 3T3-L1 cells in adipocyte assays.

Green K562-GFP with nuclear stain. Application note

A Simple and Rapid Alternative to <sup>51</sup>Chromium or Fluorescent Dye Loading for Quantification of Natural Killer Cell Activity

A flow cytometry-based assay utilizing K-562 cells stably expressing green fluorescent protein to quantify the cytotoxic activity of primary human natural killer cells.

Fluorescent orange and black primary bronchial tracheal epithelial cells. Application note

ATCC Human Bronchial-Tracheal Epithelial Cells

This study will test the ability of primary human bronchial/tracheal epithelial cells (HBECs) to differentiate into mature airway tissue using an air-liquid interface (ALI) culture model.

Gloved hand holding mini pipette over petri dish containing bacteria colonies Application note

ATCC Strains with Demonstrated Biocatalytic Ketone Reduction Capability

This study demonstrates keto-reductase enzyme activity amongst a group of 26 ATCC strains and the potential use of this activity in industrial applications aimed at outperforming traditional chemical-based approaches.

Green and blue dopaminergic Diff Tuj1 DAPI cells. Application note

Comprehensive Gene Expression Analysis and Neurotoxicity Testing of Human iPSC-derived Neural Progenitor Cells and Neurons

In this study, we examined the ability of a novel medium to stimulate neural progenitor cells (NPCs) to differentiate into multiple types of neurons.

Round, floating, blue  H1N1 influenza viruses covered in long protruding stems. Application note

Development and Evaluation of Next-Generation Sequencing Standards for Virome Research

In this study, we describe the development and quality control of the ATCC virome standards and present application data demonstrating their use throughout the different stages of a typical virome analysis workflow.

Blue DNA helix near floating translucent blue rods. Application note

Development and Evaluation of Whole Cell- and Genomic DNA-based Microbiome Reference Standards

In this study, we demonstrate that ATCC Microbiome Standards combined with the One Codex data analysis module provide a comprehensive solution for standardizing data from a wide range of sources, and generating consensus among microbiome applications and analyses.

Broken DNA helix made of purple balls. Application note

Development and Use of Synthetic Molecular Standards for Dengue Virus Serotypes 1-4

ATCC has developed four quantitated synthetic molecular standards for Dengue virus (DENV) serotypes 1-4 for use as positive controls in quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays. Read this study to explore the development and application of these molecular standards.

Gray, grainy, hexagon-shaped herpes simplex virus. Application note

Development of ATCC Molecular Standards for Human herpesvirus 1 and 2 (HSV-1 and HSV-2)

In this study, we demonstrate the utility of molecular standards for Human herpesvirus 1 and 2 in the generation of standard curves for the detection and quantification of viral load.

A light-blue, textured sphere of norovirus. Application note

Development of Improved Synthetic Molecular Standards for Norovirus Genogroups I and II

Explore the use of quantitative synthetic molecular standards for Norovirus genogroups I and II in assay development.

Grainy, blue and orange Hepatitis virus. Application note

Development of Synthetic Molecular Standards for Hepatitis B and Hepatitis C Virus

Explore the development and validation of quantitated synthetic molecular standards for Hepatitis B virus (HBV) and Hepatitis C virus (HCV) and their applications as positive controls in molecular-based assays.

Red blood cells. Application note

Differentiation and Expansion of Hematopoietic Precursor Cells from Bone Marrow-Derived CD34+ Progenitors

This study will examine the differentiation and expansion potential of bone marrow-derived (BM) CD34+ cells into erythroid cells, megakaryocytes, and myeloid precursor cells in a liquid, non-adherent culture format.

Green, red and blue organoid cells. Application note

Directed Differentiation of Gastrointestinal Epithelial Organoids Using ATCC CellMatrix Basement Membrane from Multiple Human ATCC iPSC Lines

Three human iPSC lines were tested for their ability to generate gastrointestinal organoids using defined culture media in a 2D/3D culture system using ATCC CellMatrix Basement Membrane.

an orange, sphere of Clostridium bacteria on a flat surface with lines dividing sections of it. Application note

ATCC Minis Support VITEK 2 Quality Control Testing

This study demonstrates the use of ATCC Minis as quality control strains for microbial identification on the VITEK 2 platform.

Round spheres of  fluorescent green and blue organic anion transporter proteins. Application note

Establishment and Characterization of a Kidney-Drug Interaction Model by Stably Expressing hOAT1 in HEK 293T/17 Cells

In this study, we created a HEK293T/17 cell line that stably expresses the human organic anion transporter (OAT1) and tested its transport capabilities.

Closeup of teal-blue Escherichia coli balls covered in red yard-like strings. Application note

Evaluation of GFP-labeled Control Strains for Shiga toxin-producing Escherichia coli (STEC) Assays

In this study, we evaluate the use of green fluorescent protein (GFP)-labeled strains representing several clinically relevant Shiga toxin-producing Escherichia coli (STEC) serogroups associated with foodborne disease as positive controls.

White, green and blue kidney glomeruli. Application note

Genetically Modified Renal Proximal Tubule Epithelial Cells

We created three different hTERT-immortalized human primary renal proximal tubule epithelial cells (RPTEC) that stably express the OAT1, OCT2, and OAT3 proteins. We then tested the ability of these cells to intracellularly convey known SLC protein substrates.

Green and red kidney cells. Application note

hTERT-immortalized and Primary Keratinocytes Differentiate into Epidermal Structures in 3D Organotypic Culture

In this study, we confirmed that an immortalized keratinocyte cell line, Ker-CT, is able to differentiate into organotypic skin equivalents in a 3D culture model

Green and purple vascular system. Application note

In vitro Angiogenesis Assay Using the ATCC Angio-Ready System

In vitro angiogenesis models are crucial to the study of blood vessel growth. Here, we report that an assay-ready, pre-mixed co-culture of human telomerase-immortalized (hTERT) human aortic endothelial cells that express green fluorescent protein (TeloHAEC-GFP) and hTERT mesenchymal stem cells (ACS52telo) produce a vascular network reminiscent of in vivo angiogenesis.

White blood cells. Application note

In Vitro Differentiation of Macrophages and Dendritic Cells from Primary Human CD14+ Monocytes

This study will demonstrate the differentiation potential of cryopreserved primary human CD14+ monocytes towards macrophage and dendritic cell lineages for applications such as cancer immunology, vaccine development, inflammation, bone disease, immunosuppression, disease resolution, and tissue repair studies.

DNA helix made of green and yellow puffy balls. Application note

Microbial Genome Databases

As part of the Enhanced Authentication Initiative, we identified the key challenges regarding existing microbial genome databases and developed a solution for improving the quality of reference genome sequences.

Petri dish filled with bright-blue and green media and cells in a line and spot pattern. Application note

Novel Fluorescent Reporters for Studying Host-Pathogen Interactions

We have developed green fluorescent protein (GFP)-labled strains of various Gram-negative opportunistic pathogens from the ATCC collection. Here, we demonstrate the use of these labeled strains in studying bacterial pathogenesis, host-pathogen interactions, and compound screening assays.

Green, pink and blue organoid cells. Application note

Protocol for the Thawing, Expansion, and Cryopreservation of Mouse Small Intestinal Organoids

Here, we describe a protocol for the initiation, culture, and subsequent cryopreservation of mouse small intestinal organoids starting from previously cryopreserved primary tissue–derived organoids.

Blue and red human umbilical vein endothelial cells. Application note

Telomerase-immortalized Microvascular Endothelial (TIME) Cells

In this study, we demonstrate that TIME cells maintain their normal growth rate and endothelial character at late passage when grown in ATCC’s VCBM-VEGF medium, but not in the previously recommended Lonza EGM™-2 MV BulletKit medium.

Pink and orange lung cancer cells. Application note

Generation of an EML4-ALK Fusion NSCLC Isogenic Cell Line Relevant for Drug Discovery and Development

An isogenic cell line was created to model cancer patients with the echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) fusion oncogene, a key oncogenic driver, and then tested for its sensitivity to known selective inhibitors of ALK.

Green and red vero cell. Application note

Generation of Cell Lines Capable of Producing High-Titer Viral Stocks for Use in Vaccine Manufacture and Gene Therapy

In this study, we used CRISPR/Cas9 gene-editing technology to create cell lines capable of producing model clinical viruses and adeno-associated viruses at titers much higher than the parental cell lines, providing an efficient method for biopharmaceutical companies to increase production while reducing associated costs.

Female ATCC scientist in lab coat, safety glasses, and gloves, looking at media in a flask using an inverted microscope. Application note

Tips for Successful Sub-Culturing: Dissociation Method

Discover how to tailor your dissociation method to your cell line to ensure successful sub-culture.

White box with ATCC printed on the side, labeled "universal mycoplasma detection kit," with capped vials next to it. Application note

Universal Mycoplasma Detection Kit

To facilitate the rapid and reliable detection of Mycoplasma in cell culture, ATCC offers the PCR-based Universal Mycoplasma Detection Kit. Explore how to use the kit to check your cultures for mycoplasma contamination.

Green stem attached to a cluster of small, round, purple Aspergillus fumigatus. Application note

Use of the ATCC <em>Aspergillus Fumigatus</em> Drug Testing Panel in Antifungal Drug Testing

Explore the development and application of a panel of A. fumigatus strains exhibiting various levels of sensitivity to common antifungal drugs.

Blue and white parts of RNA strand. Application note

Use of Quantitative Mycoplasma DNA Controls in Evaluating Sensitivity

Learn how ATCC-designed quantitative mycoplasma DNA controls can be used to evaluate the sensitivity of two different molecular-based detection systems.

Flat green, rod-shaped Vibrio vulnificus bacteria. Application note

<em>Vibrio Campbellii</em> Quorum Sensing

This study will demonstrate the use of the ATCC Vibrio campbellii Panel as a non-pathogenic model for AI-2-based quorum sensing pathways.


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