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Application Notes

Red tipped multi-channel pipettor above multicolored well plates.
Advanced biological models and credible reference standards enable greater specificity and functionality to the researcher’s toolkit. Browse our growing collection of application notes for access to in-depth data on the development and validation of these products and to explore proof-of-concept studies focused on their various applications.
Immune checkpoint inhibitors: Interaction between PD-1 (blue) on a T-cell and PD-L1 (red) on a cancer cell blocked by therapeutic antibodies Application note

GAS-LUC2 Reporter Cell Lines for Immune Checkpoint Drug Screening in Solid Tumors

This study demonstrates the use of three robust, responsive, and reproducible GAS-Luc2 reporter solid tumor cell lines for the assessment of T cell and other immune/tumor microenvironmental cell-mediated immune responses triggered by PD-1/PD-L1, CD-155/TIGIT, or B7-H3 (CD276) immune checkpoints.

Blue and green mouse embryo fibroblast. Application note

3T3-L1 Cells Lose Contact Inhibition and Adipogenic Potential When Maintained in Culture at Confluence

This study determines if 3T3-L1 adipocyte differentiation is dependent on contact inhibition and tests the OP9 cell line as a possible substitute for 3T3-L1 cells in adipocyte assays.

an orange, sphere of Clostridium bacteria on a flat surface with lines dividing sections of it. Application note

ATCC Minis Support VITEK 2 Quality Control Testing

This study demonstrates the use of ATCC Minis as quality control strains for microbial identification on the VITEK 2 platform.

Green K562-GFP with nuclear stain. Application note

A Simple and Rapid Alternative to 51Chromium or Fluorescent Dye Loading for Quantification of Natural Killer Cell Activity

A flow cytometry-based assay using K-562 cells stably expressing green fluorescent protein to quantify the cytotoxic activity of primary human natural killer cells.

Fluorescent orange and black primary bronchial tracheal epithelial cells. Application note

ATCC Human Bronchial-Tracheal Epithelial Cells

This study will test the ability of primary human bronchial/tracheal epithelial cells (HBECs) to differentiate into mature airway tissue using an air-liquid interface (ALI) culture model.

Long, bright green rods and balls of  penicillium mold. Application note

ATCC Strains with Demonstrated Biocatalytic Ketone Reduction Capability

This study demonstrates keto-reductase enzyme activity amongst a group of 26 ATCC strains and the potential use of this activity in industrial applications aimed at outperforming traditional chemical-based approaches.

3D Illustration Concept of Human Respiratory System Lungs Anatomy Application note

Comparing Toxicological Responses Between ALI-Incubated Primary Airway Epithelial Cells and Undifferentiated Counterparts

In this application note, we show that self-constructed airway models may serve as useful tools future airway toxicity research.

Green and blue dopaminergic Diff Tuj1 DAPI cells. Application note

Comprehensive Gene Expression Analysis and Neurotoxicity Testing of Human iPSC-derived Neural Progenitor Cells and Neurons

This study examines the ability of a novel medium to stimulate neural progenitor cells to differentiate into multiple types of neurons.

Broken DNA helix made of purple balls. Application note

Development and Use of Synthetic Molecular Standards for Dengue Virus Serotypes 1-4

ATCC has developed 4 quantitated synthetic molecular standards for Dengue virus (DENV) serotypes 1-4 for use as positive controls in qRT-PCR assays. Read this study to explore the development and application of these molecular standards.

Monkeypox virus Application note

Development and Validation of a Quantitative Synthetic Molecular Standard for Monkeypox virus

In this study, ATCC designed and developed a quantitative synthetic molecular standard for Monkeypox virus to serve as a safe and reliable positive control material when conducting diagnostics assays.

Green, orange, red and blue organoid cells. Application note

Directed Differentiation of Gastrointestinal Epithelial Organoids

Three human iPSC lines were tested for their ability to generate gastrointestinal organoids using defined culture media in a 2-D/3-D culture system using ATCC Cell Basement Membrane.

Neural progenitor cell derived neurosphere Application note

Establishment of 3-D Neurosphere Culture from Human iPSC-Derived Neural Progenitor Cells

This report describes a straightforward and robust method of generating neurospheres from neural progenitor cells

Pink and orange lung cancer cells. Application note

Generation of an EML4-ALK Fusion NSCLC Isogenic Cell Line Relevant for Drug Discovery and Development

An isogenic cell line was created to model cancer patients with the echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) fusion oncogene, a key oncogenic driver, and then tested for its sensitivity to known selective inhibitors of ALK.

Long, thin, red, ebola rods stuck to a porous black-blue surface. Application note

Generation of Cell Lines Capable of Producing High-titer Viral Stocks for Use in Vaccine Manufacture and Gene Therapy

In this study, we used CRISPR/Cas9 gene-editing technology to create cell lines capable of producing model clinical viruses and adeno-associated viruses at titers much higher than the parental cell lines, providing an efficient method for biopharmaceutical companies to increase production while reducing associated costs.

White, green and blue kidney glomeruli. Application note

Genetically Modified Renal Proximal Tubule Epithelial Cells

We created three different hTERT-immortalized human primary renal proximal tubule epithelial cells (RPTEC) that stably express the OAT1, OCT2, and OAT3 proteins. We then tested the ability of these cells to intracellularly convey known SLC protein substrates.

Green and red kidney cells. Application note

hTERT-immortalized and Primary Keratinocytes Differentiate into Epidermal Structures in 3-D Organotypic Culture

This study shows that an immortalized keratinocyte cell line, Ker-CT, is able to differentiate into organotypic skin equivalents in a 3-D culture model

Fluorescent red, lime green, purple, and blue dendritic cells. Application note

In Vitro Differentiation of Macrophages and Dendritic Cells

This study will demonstrate the differentiation potential of cryopreserved primary human CD14+ monocytes towards macrophage and dendritic cell lineages for applications including cancer immunology and vaccine development,

DNA helix made of green and yellow puffy balls. Application note

Microbial Genome Databases

As part of the Enhanced Authentication Initiative, we identified the key challenges regarding existing microbial genome databases and developed a solution for improving the quality of reference genome sequences.

Petri dish filled with bright-blue and green media and cells in a line and spot pattern. Application note

Novel Fluorescent Reporters for Studying Host-pathogen Interactions

We have developed green fluorescent protein (GFP)-labled strains of various Gram-negative opportunistic pathogens from the ATCC collection. Here, we demonstrate the use of these labeled strains in studying bacterial pathogenesis, host-pathogen interactions, and compound screening assays.

Green, pink and blue organoid cells. Application note

Protocol for the Thawing, Expansion, and Cryopreservation of Mouse Small Intestinal Organoids

Here, we describe a protocol for the initiation, culture, and subsequent cryopreservation of mouse small intestinal organoids starting from previously cryopreserved primary tissue–derived organoids.

Green stem attached to a cluster of small, round, purple Aspergillus fumigatus. Application note

Use of the ATCC Aspergillus fumigatus Drug Testing Panel in Antifungal Drug Testing

Explore the development and application of a panel of A. fumigatus strains exhibiting various levels of sensitivity to common antifungal drugs.

Blue and white parts of RNA strand. Application note

Use of Quantitative Mycoplasma DNA Controls in Evaluating Sensitivity

Learn how ATCC-designed quantitative mycoplasma DNA controls can be used to evaluate the sensitivity of two different molecular-based detection systems.

alcian blue ALI airway diagonal Application note

Evaluating Airway ALI Model Fabrication Methods

This report showcases an optimal method of fabricating airway models consisting of human bronchial tracheal epithelial cells grown under air-liquid interface (ALI).

Purple floating rods of  Escherichia coli bacteria. Application note

Evaluation of GFP-labeled Control Strains for Shiga Toxin-producing Escherichia coli STEC Assays

In this study, we evaluate the use of green fluorescent protein (GFP)-labeled strains representing several clinically relevant Shiga toxin-producing Escherichia coli (STEC) serogroups associated with foodborne disease as positive controls.

Light blue and dark blue DNA helix. Application note

Development and Evaluation of Whole Cell- and Genomic DNA-based NGS Standards

In this study, we demonstrate that ATCC NGS Standards combined with the One Codex data analysis module provide a comprehensive solution for standardizing data from a wide range of sources, and generating consensus among microbiome applications and analyses.

Gray, grainy, hexagon-shaped herpes simplex virus. Application note

Development of ATCC Molecular Standards for Human Herpesvirus

In this study, we demonstrate the utility of molecular standards for Human herpesvirus 1 and 2 in the generation of standard curves for the detection and quantification of viral load.

Light blue and dark blue DNA helix. Application note

Development of Improved Synthetic Molecular Standards for Norovirus Genogroups I and II

Explore the use of quantitative synthetic molecular standards for Norovirus genogroups I and II in assay development.

Purple, fragmented DNA double helix strand on a black background. Application note

Development of Synthetic Molecular Standards for Hepatitis B and Hepatitis C Virus

Explore the development and validation of quantitated synthetic molecular standards for Hepatitis B virus (HBV) and Hepatitis C virus (HCV) and their applications as positive controls in molecular-based assays.

Green K562-GFP with nuclear stain. Application note

Differentiation and expansion of hematopoietic precursor cells from bone-marrow derived CD34+ progenitors

This study will examine the differentiation and expansion potential of bone marrow-derived (BM) CD34+ cells into erythroid cells, megakaryocytes, and myeloid precursor cells in a liquid, non-adherent culture format.

Closeup of purple virus spheres made up of long, iridescent spikes. Application note

Development and Evaluation of Next-generation Sequencing Standards for Virome Research

In this study, we describe the development and quality control of the ATCC virome standards and present application data demonstrating their use throughout the different stages of a typical virome analysis workflow.

Green and blue human embryonic kidney cells. Application note

Establishment and Characterization of a Kidney-drug Interaction Model

In this study, we created a HEK293T/17 cell line that stably expresses the human organic anion transporter (OAT1) and tested its transport capabilities.

CAR-T cells, CAR-Target, CD19, CD20, HER2 Application note

Luciferase Reporter Cancer Cell Lines Facilitate CAR-T Development

This report describes ATCC luciferase reporter solid and liquid tumor cell lines that express tumor antigens such as CD19, CD20, and HER2. Learn how you can incorporate these target cells into your T-Cell cytotoxicity assays to enable your breakthroughs in immuno-oncology.

Purple, red and black human umbilical vein endothelial cells. Application note

Telomerase-immortalized Microvascular Endothelial TIME Cells

In this study, we demonstrate that TIME cells maintain their normal growth rate and endothelial character at late passage when grown in ATCC’s VCBM-VEGF medium, but not in the previously recommended Lonza EGM™-2 MV BulletKit medium.

Eye dropper with drop of clear liquid, over top of row of test tubes. Application note

Tips for Successful Subculturing: Dissociation Method

Discover how to tailor your dissociation method to your cell line to ensure successful subculture.

Gold, bean-shaped pods of Mycoplasma pneumoniae. Application note

Universal Mycoplasma Detection Kit

To facilitate the rapid and reliable detection of Mycoplasma in cell culture, ATCC offers the PCR-based Universal Mycoplasma Detection Kit. Explore how to use the kit to check your cultures for mycoplasma contamination.

Purple floating rods of  Escherichia coli bacteria. Application note

Use of Recombinant Bacteria with Unique Tags as Spike-in Controls for Microbiome Studies

In this application note, we describe the construction and application of ATCC Spike-in Standards as a tool for quantitative metagenomic analysis.

Eye dropper with drop of clear liquid, over top of row of test tubes. Application note

Vibrio campbellii Quorum Sensing

This study will demonstrate the use of the ATCC Vibrio campbellii Panel as a non-pathogenic model for AI-2-based quorum sensing pathways.


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