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Microbial Genome Databases

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Existing challenges and the need for authenticated reference genomes

Authors: Juan Lopera, PhD; Andrew Frank, MS; Anna McCluskey BS; Stephen King, MS; Samantha Fenn, BS; Karin Kindig, MS; Marco Riojas, PhD; Jung-Woo Sohn, PhD; Holly Sadural, BS; Benton Briana, BS; and Cara Wilder, PhD

Survey of ATCC Genome Sequences in Public Genome Databases
Evaluation of ATCC Genome Sequences From Public Databases
Development and Evaluation of Hybrid De Novo Bacterial Assembly


While recent technological advancements have enabled the generation of vast amounts of whole-genome sequencing data, publicly available reference genomes often lack quality, completeness, authenticity, accuracy, and traceability. The reliability of these data is further called into question as they may have been generated using untraceable cultures and older methodologies. In the following study, we surveyed the status of ATCC bacterial genome sequences in public databases and described the implementation of a genome sequencing workflow designed to provide reference-quality whole-genome sequences that are derived from authenticated ATCC materials.

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The advancement and accessibility of next-generation sequencing (NGS), cloud computing, and sequence analysis tools have rapidly transformed microbiological research by opening up applications in the areas of clinical diagnostics, drug discovery, public health, microbiome research, antimicrobial resistance studies, and industrial and environmental microbiology.1-3 Many of these NGS-based applications have relied on the availability of high-quality assembled and annotated genome sequences in public databases to serve as references and control for bioinformatic analyses.4-6 However, despite the large number of existing microbial genome sequences in various public databases, the quality, completeness, authenticity, accuracy, and traceability of some genomic data are frequently questionable as they could have been generated by various researchers using non-authenticated cultures and older sequencing and analysis technologies. Further, the lack of standardized methodologies for best practices during the sequencing and assembly of reference genomes exacerbates the underlying problems.

For researchers to accurately interpret their results and make insightful correlations with in silico models, it is essential that they have access to reliable genomic information tied back to authenticated, fully characterized materials of known and reliable provenance. Therefore, as part of our initiative to enhance the authentication of our products, we have identified the key challenges regarding existing microbial genome databases and have developed a solution for improving the quality of reference genome sequences. In the following study, our results demonstrated that there is no significant representation of ATCC strains with genome sequences available in public databases, and even fewer of these strains have complete circularized chromosome and plasmids. Here, we examine those results in detail and discuss the development and application of our standardized end-to-end sequencing and assembly workflow for producing reference-quality genome sequences (Figure 1).

Comprehensive ATCC bacterial whole-genome sequencing workflow.

Figure 1. Comprehensive ATCC bacterial whole-genome sequencing workflow.

Survey of ATCC genome sequences in public genome databases

A reference genome is a high-quality sequence published in a database that provides a representative example of a species; these sequences are reviewed and validated extensively.7,8 Today, there are multiple distinct microbial genomic resources, tools, and databases publicly available in internet portals.9-13 In this study, we focused the survey of bacterial genome sequences on those identified by the depositor of the sequence as ATCC materials in two important public genome sequence databases: Microbial Genomes (NCBI-NIH) and Ensembl Bacteria (EMBL-EBI). The purpose of the study was to collect information about the number and assembly status (scaffold, contigs, and complete bacterial chromosomes and plasmids) of published ATCC strains in two of the most frequently used databases that are of interest for microbiology research (Table 1). Here, database records containing the keyword “ATCC” were extracted and compared to the entire collection of database entries. Records in the Microbial Genomes database provided the complete information of assembly level (contigs, scaffolds, chromosomes, and plasmids) (Table 1). Since records in the Ensembl Bacteria database do not directly present the information with assembly level information, a Python script that extracts the genome assembly statistics based on the accession number was written and run to generate the data (Table 1).

Of the records evaluated in the public databases, we identified a total of 1,807 (1.1%) ATCC prokaryote genomes sequences classified as RefSeq in the Microbial Genomes database and 715 (1.6%) ATCC strains in the Ensembl Bacteria database (Table 1). Further, we identified that the surveyed genome sequences were primarily characterized to be incomplete genome drafts consistent of multiple noncontiguous scaffolds or contigs. Specifically, 27.7% of ATCC strains in the Microbial Genomes database and 10.9% ATCC strains from the Ensembl Bacteria database were identified as complete genomes, whereas 72.3% and 89.1% of the ATCC strains in the Microbial Genomes and Ensembl Bacteria databases, respectively, were genome drafts with fragmented genome scaffolds or contigs (Table 1, Figure 2). We also observed that in the Microbial Genomes database approximately 12% of ATCC strains had more than one genome report available. These results demonstrate that while there are a relatively large number of ATCC genome sequences available in multiple public databases, there is a deficiency of complete ATCC circularized genome and plasmids sequences (Table 1, Figure 2).

Table 1. Summary of the microbial genome database survey results

Database # of genome sequences (%) Contigs or scaffolds (%) Complete genome or chromosome (%) Genome with plasmids (%)
Microbial Genomes (NCBI-NIH) (RefSeq prokaryote database) 165,807* (80.2%) 149,171 (90.0%) 16,636 (10.0%) 6,333 (3.8%)
ATCC strains in Microbial Genomes (NCBI-NIH) (RefSeq prokaryote databases) 1,807 (1.1%) 1307 (72.3%) 500 (27.7%) 193 (10.7%)
Ensembl Bacteria (EMBL-EBI) 44,011* (96.9%) 39,203 (89.1%) 4,808 (10.9%) NA**
ATCC strains in Ensembl Bacteria (EMBL-EBI) 715 (1.6%) 521 (72.9%) 194 (27.1%) NA**

*Microbial Genomes database = total 206,660 records evaluated with 165,807 RefSeq hits; Ensembl Bacteria database = 45,438 records evaluated, with 44,048 specific bacteria hits (1,390 record from Ensembl Bacteria database were identified as fungal or viral organisms).
**NA, not applicable; data was not available in the databases

 Survey profiles of ATCC genomes organized by assembly status in the (A) Microbial Genomes and (B) Ensembl Bacteria.

Figure 2. Survey profiles of ATCC genomes organized by assembly status in the (A) Microbial Genomes and (B) Ensembl Bacteria. Note: EMBL-EBI does not differentiate between contigs and scaffolds and does not include plasmid information.

Evaluation of ATCC genome sequences from public databases

To evaluate the quality of published ATCC genomes from public databases and to demonstrate the need of credible reference-quality genomes, we analyzed a select group of strains via sequencing. Here, 100 bacterial strains identified in our genome database survey as having complete assemblies were randomly selected for analysis. Then, using nucleic acids extracted from low passage ATCC bacterial cultures, we re-sequenced the selected strains and analyzed each sequence using customized reference-based assembly (short-read alignment/mapping to published genome sequences) and hybrid de novo assembly (short- and long-read analysis) workflows.

Preparation and quality control of DNA templates

Unlike many of the bacterial genome sequences deposited in public databases, we began our genome sequencing efforts with the comprehensive traceability of ATCC authenticated strains. This allows us to validate the source of the bacterial culture and genomic DNA while linking to vital metadata, thus enabling downstream references and support for analyses. Briefly, before we engaged in the quality assessment of ATCC genomes present in public databases, we carefully reviewed the classification of the bacterial cultures and evaluated the quality and purity of the DNA template used for NGS sequencing. To facilitate the successful NGS library preparation for multiple sequencing platforms (long- and short-read sequences), we used either input DNA obtained directly from authenticated and fully characterized ATCC nucleic acids from our repository or DNA with high molecular weight (NGS-ready DNA) and fragment sizes bigger than 20 kb that were extracted directly from our cultures. The quality and quantity of the DNA used in this study were measured via a DNA analyzer (Agilent) and a fluorescent dye-based method PicoGreen, respectively (Figure 3, Table 2).

Quality assessment for NGS-ready DNA used in this study. Quality assessment for NGS-ready DNA used in this study.

Figure 3. Quality assessment for NGS-ready DNA used in this study. The fragment size graph obtained from the Agilent Fragment Analyzer platform demonstrates the size distribution of total DNA. The graphs depict examples of DNA quality assessments for a gram-negative (A) Escherichia coli (ATCC 8739DX) and gram-positive strain (B) Staphylococcus aureus (ATCC 6538DX), respectively.

Table 2. Summary of DNA quality and quantity measurements before NGS

ATCC No. Species PicoGreen (ng/µL) A260/A280 DNA fragment size (range)**
8739DX* Escherichia coli 101.9 1.92 49.5 kb (1.5 – >60 kb)
13048DX* Klebsiella aerogenes 98.1 1.86 49.5 kb (1.6 – >60 kb)
11828DX* Cutibacterium acnes 197.7 1.84 29.8 kb (0.8 – >60 kb)
6538DX* Staphylococcus aureus 97.8 1.85 32.9 kb (2.7 – >60 kb)
BAA-2797DX* Pseudomonas aeruginosa 153.3 1.99 44.1 kb (1.1 – >60 kb)
824D-5 Clostridium acetobutylicum 73.8 2.05 12.5 kb (4.6 – 57.8 kb)
6538D-5 Staphylococcus aureus 37.1 2.00 26.2 kb (6.9 – >60 kb)
27774D-5 Desulfovibrio desulfuricans 69.2 1.99 58.5 kb (13.3 – >60 kb)
11842D-5 Lactobacillus delbrueckii 64.8 2.02 41.9 kb (6.1 – >60 kb)
15697D-5 Bifidobacterium longum 76.2 1.95 51.3 kb (10.5 – >60 kb)

*NGS-ready DNA
**DNA fragment size represents the main peak reported by the fragment analyzer

High-quality NGS sequences

Because NGS has emerged as a sensitive and precise tool for microbial characterization, diagnostics, and discovery, assessing the quality of the raw NGS data has become indispensable for ensuring the credibility of assemblies and the annotation of reference genomes.6,14,15 In public databases, the general submission process for raw sequence data requires some data quality information. Sequence Read Archive (SRA) requires supporting per-base quality scores for all submitted sequences. For the genome assemblies, whole-genome sequencing (WGS) submission requests the base-level quality for which files are not strictly required. However, there are not any standardized sequence quality thresholds that measure or regulate the excellence of the genomic information deposited in public databases.15-17 For this reason, we have developed and implemented a rigorous quality control protocol that includes the analysis of raw sequence quality scores and removal (trimming) of low-quality segments and undefined nucleotides as well as a read-based contamination quality control via the One Codex database (Figure 4). For additional details on the quality control processes we have implemented, see the ATCC Genome Portal Technical Document.

ATCC’s bacterial genome sequencing quality control. ATCC’s bacterial genome sequencing quality control. ATCC’s bacterial genome sequencing quality control.

Figure 4. ATCC’s bacterial genome sequencing quality control. The dashed line indicates the quality score cutoff used for each sequencing technology. (A) Quality of Illumina reads. (B) Length distribution of reads from the Oxford Nanopore Technologies (ONT) platform. This approach ensures the longest, highest-quality reads are used for assembly. Thus, the lengths of ONT raw sequence and quality scores were evaluated by measuring read lengths N50 (>5000kb), quality scores (>10), and total yield of sequence runs. (C) Sample composition describes NGS composition by aligning each individual read to a reference database. We use the One Codex microbial genomics platform to perform read-level, k-mer–based taxonomic classification and estimation of strain abundances on our processed Illumina read sets.


Reference-based analysis and variant calling

We evaluated the level of genetic variation between published sequences and NGS sequences obtained directly from ATCC cultures. For 100 sequences identified as ATCC materials in public databases, we ran a reference-based analysis tool on our short reads to identify single nucleotide variations (SNVs) and indels (small insertion/deletion). Briefly, high-accuracy and high-coverage (>100x) Illumina sequences (MiSeq PE 2x300) from ATCC DNAs corresponding to the selected strains were aligned and mapped to published reference assemblies. The genome variants threshold was fixed to a variant average coverage greater than 100x. To validate our results, all of the sequences were first validated by the previously described quality control filter, and then six random strains were sequenced and analyzed in duplicate (Table 4).

Our results demonstrated that approximately 33% of the 100 strains evaluated have fewer than 50 variants (SNVs and indels); 14 strains showed low sequence variation with fewer than 5 variants, and 8 strains showed large sequence variation with more than 500 variants detected. When SNVs and indels were evaluated separately, we found that 18% of the strains exhibited more than 50 SNVs and 37% of the public genomes displayed more than 25 indels. Interestingly, 14 of the selected ATCC strains analyzed from public databases showed more than one assembly record, and three of these contained a different number of plasmids reported between the two separate assembles from the same strain identification (Table 3). Overall, we found that a considerable number of sequenced ATCC strains contain significant variations as compared to their public database counterparts. Without the accurate metadata and sample traceability, it is difficult to identify the source of the variation. In some cases, these variations may be attributable to the incorrect identification of the ATCC isolate before the sequence is submitted (eg, sequencing from a strain other than the intended ATCC strain). In other cases, the variations may have been caused by differences in strain propagation, DNA extraction, sequencing quality, or downstream assembly analysis, which could influence the overall quality of data in historical sequencing databases.

Table 3. Summary of variant call analysis for strains with more than one database record.

Species ATCC No. Existing Reference Genomes NCBI assembly level (plasmids*) # of SNPs # of indels Average coverage (variants)
Acinetobacter baumannii 17978 GCA_001593425.2 Complete genome 14 5 210.1
Acinetobacter baumannii 17978 GCA_000015425.1 Complete genome (2) 118 656 152.7
Porphyromonas gingivalis 33277 GCA_000010505.1 Complete genome 20 7 319.5
Porphyromonas gingivalis 33277 GCA_002892575.1 Complete genome 24 8 323.8
Staphylococcus epidermidis 12228 GCA_002215535.1 Complete genome (5) 56,346 2,328 181.2
Staphylococcus epidermidis 12228 GCA_000007645.1 Complete genome (6) 66 35 129.5
Fusobacterium nucleatum 25586 GCA_003019295.1 Complete genome 29 14 310.4
Fusobacterium nucleatum 25586 GCA_000007325.1 Complete genome 49 22 289.7
Corynebacterium glutamicum 13032 GCA_000011325.1 Complete genome 18 2 216.7
Corynebacterium glutamicum 13032 GCA_000196335.1 Complete genome 88 62 175.0
Escherichia coli 8739 GCA_000019385.1 Complete genome 24 0 175.9
Escherichia coli 8739 GCA_003591595.1 Complete genome 5 14 179.8
Bifidobacterium longum 15697 GCA_000020425.1 Complete genome 14 7 336.1
Bifidobacterium longum 15697 GCA_000269965.1 Complete genome 5 5 312.6
Vibrio campbellii BAA-1116 GCA_000464435.1 Complete genome [2 chr](1) 198 336 143.0
Vibrio campbellii BAA-1116 GCA_000017705.1 Complete genome [2 chr](1) 26 47 107.3
Bacillus licheniformis 14580 GCA_000008425.1 Complete genome 17 4 174.4
Bacillus licheniformis 14580 GCA_000011645.1 Complete genome 14 5 201.7
Vibrio natriegens 14048 GCA_001456255.1 Complete genome [2 chr] 4 10 152.3
Vibrio natriegens 14048 GCA_001680025.1 Complete genome [2 chr] 21 50 70.63

*Number in parentheses represent the number of plasmids reported in NCBI assembly report.


To support the sequence variation observed in ATCC genome sequences from public databases and assess the quality of our sequences, we performed independent short-read sequencing in duplicate using different experimental variables (Table 4). We then measured the reproducibility of our analysis via the number of SNVs and indels detected and the level of variant coverage observed.


Table 4. Summary of the reference base mapping analysis from multiple datasets.

Test Species ATCC No. Reference Genome Analysis # of SNPs # of indels Number of variants Variant coverage
A Mycoplasma hominis 23114 GCA_000085865.1 Preparation 1 14 10 24 1042.1
A Mycoplasma hominis 23114 GCA_000085865.1 Preparation 2 14 10 24 900.0
A Cutibacterium acnes 11828 GCA_000231215.1 Preparation 1 28 37 65 121.1
A Cutibacterium acnes 11828 GCA_000231215.1 Preparation 2 28 39 67 128.6
B Clostridium acetobutylicum 824D-5 GCA_000008765.1 Kit 1 171 55 226 95.9
B Clostridium acetobutylicum 824D-5 GCA_000008765.1 Kit 2 170 55 225 202.0
B Aeromonas hydrophila 7966D-5 GCA_000014805.1 Kit 1 1 1 2 216.8
B Aeromonas hydrophila 7966D-5 GCA_000014805.1 Kit 2 1 1 2 203.0
C Escherichia coli 700926 GCA_000005845.2 Extraction 1 0 1 1 137.0
C Escherichia coli 700926 GCA_000005845.2 Extraction 2 0 1 1
C Streptococcus pyogenes 19615 GCA_000743015.1 Extraction 1 2 44 46 314.2
C Streptococcus pyogenes 19615 GCA_000743015.1 Extraction 2 2 41 43 460.2

Test A: Same DNA sequenced using two different DNA preparations
Test B: Same DNA sequenced with two different library kits
Test C: Same strain extracted with two different methods

Development and evaluation of hybrid de novo bacterial assembly

The results from the read alignment and mapping for variant detection in the ATCC published genomes demonstrated a diverse range of errors that impact the integrity of multiple reference genomes in public databases (Table 3). Therefore, it is essential that more sequence quality control and a standardized assembly approach are used to evaluate reference genome sequences.18 Several tools and NGS technologies used for de novo genome assembly have demonstrated the advantages of using a hybrid approach for the generation of precise genome sequences.19-21 With the goal of producing complete and circularized bacterial chromosomes and plasmids, and to provide a precise reference standard sequences from strains of our microbiology collection, we developed and standardized a hybrid de novo assembly and annotation bioinformatics pipeline. To minimize the potential effect of base call errors reported for ONT sequences,22 we developed a quality control protocol to trim and filter sequences by size and quality with fixed thresholds (Figure 4B).

Briefly, DNA from the ATCC collection and the extracted NGS-ready DNA were sequenced using both short-read and long-read NGS platforms. To continue the implementation of the best practices for our genome analysis workflow, and to reduce biases related to read length, sequence quality, coverage, and genome complexity like % GC and repeat regions, we standardized a method for sequencing DNA by using two NGS technologies and several instruments. First, this dual sequencing approach ensures the generation of high-quality contiguous and circular genome contigs with accurate base call and error polishing via high-quality Illumina short read coverage (median Q score, all bases > 30 and coverage threshold > 100x) and bacterial chromosome scaffolding and circularization with quality-filtered ultra-long reads obtained by Oxford Nanopore sequencing (minimum mean Q score, per reads >10, and minimum reads length > 5kb). Second, to guarantee a correct and complete de novo genome and to verify the taxonomic classification of the new reference ATCC genome sequences, we annotated and assessed the quality of genomes produced by our analysis pipeline by using previously published and certified bioinformatic tools (for additional details see ATCC Genome Portal Technical Document). Through this hybrid de novo assembly approach, we were able to generate complete circular chromosomes from ATCC certified strains, and we identified a diverse number of assembly errors (eg, single variants and chromosomal rearrangements) in the ATCC genome sequences from public databases (Figure 5, Table 5).

Table 5. Summary of metadata and quality of genome assemblies from public databases.

ATCC No. GenBank assembly GenBank seq. platform Year published to GenBank # of variants between ATCC and GenBank assemblies Average variant coverage Structural variation detected
8739 GCA_000019385.1 Not reported 2008 20 159.9X No
12228 GCA_002215535.1 PacBio 2017 58,674 181.2X Yes
13048 GCA_003417445.1 Ion Torrent 2018 736 171.4X n/a
14028 GCA_003864015.1 PacBio 2018 81 102.5X Yes
17978 GCA_001593425.2 Illumina MiSeq 2016 21 204.0X Yes


Evaluation of genome sequences from public databases.

Evaluation of genome sequences from public databases.

Figure 5. Evaluation of genome sequences from public databases. (A) Pairwise comparisons between select assembled ATCC genomes and their GenBank counterpart for a variety of assembly metrics. ATCC genomes (purple) show comparable or better assembly metrics than publicly available genomes (green). CDSs is coding sequences; N50 is the size of the shortest contig when 50% of the genome is contained in contigs of the same size or larger. (B) MUMmer24 alignment of ATCC de novo genome assembly of ATCC 12228 versus GenBank RefSeq genome assembly GCA_002215535.1, and plasmid alignments. Results are indicative of substantial structural variation and no complete matching plasmids between assemblies.


Quality control of genome assemblies

We used CheckM23 and taxonomic analysis to validate the quality of the assembly and the species designation (for additional details see ATCC Genome Portal Technical Document). Additionally, to support the quality of our analysis, we compared the ATCC genome assemblies developed using our workflow with the de novo hybrid assemblies (Nanopore and PacBio) produced by an external and certified third-party sequence facility that used same ATCC cultures. Here, twelve ATCC original cultures were extracted by an external laboratory and then sequenced and assembled using its analysis pipeline. To review the quality of the assemblies, we performed a WGS comparison between multiple datasets of the genome sequences obtained from our pipeline and the genome sequences assemblies obtained by the external lab (Figure 6, Table 6). Together, these results validate the reproducibility and confidence of our study and support the construction of an authenticated reference genome database.

Examples of whole-genome sequence alignments between ATCC assemblies and external source assemblies.

Figure 6. Examples of whole-genome sequence alignments between ATCC assemblies and external source assemblies. (A) Whole-genome sequence alignments for Escherichia coli (ATCC 8739). (B) Whole-genome sequence alignments for Bacillus subtilis (ATCC 6633). Solid color blocks represent high sequence homology and structural similarity between two separated assemblies. Figures were generated using Mauve.25

Table 6. Summary of the de novo genome assemblies from multiple databases

Species ATCC No. Sequence dataset Total consensus (Mbp) # of contigs (circular) N50 (Mbp) % GC
Bacillus subtilis 6633 Extraction 1 4.041 1 (1) 4.041 43.9
Bacillus subtilis 6633 Extraction 2 4.041 1 (1) 4.041 43.9
Bacillus subtilis 6633 Extraction 3 4.045 1 (1) 4.045 43.9
Bacillus subtilis 6633 Extraction 4* 4.045 1 (1) 4.045 43.9
Escherichia coli 8739 Extraction 1 4.747 1 (1) 4.747 50.9
Escherichia coli 8739 Extraction 2 4.746 1 (1) 4.746 50.9
Escherichia coli 8739 Extraction 3* 4.746 1 (1) 4.746 50.9
Staphylococcus aureus 6538 Extraction 1 2.800 2 (2) 2.772 32.9
Staphylococcus aureus 6538 Extraction 2 2.800 2 (2) 2.772 32.9
Staphylococcus aureus 6538 Extraction 3* 2.800 2 (2) 2.772 32.9

*Datasets obtained for DNA extraction, sequencing, and analysis from an external lab using ATCC Genuine Cultures


Finally, we evaluated several available annotation packages to select the most efficient, precise, and complete tool to provide the gene annotation for the final ATCC genome assemblies. Here, the genome assembly of Escherichia coli K-12 (ATCC 12435) was annotated using four different tools: Prokka, PGAP, EcoCyc (a combination of bioinformatics tools and manual curation by scientists), and AMG (an in-house advanced microbial genome annotation pipeline).26-28 In this analysis, we considered EcoCyc to be the “gold standard” for bacterial genome annotation. In general, the best results and most complete features were produced for PGAP (Table 7). The results suggest equivalent outputs of Prokka and PGAP and correct features specified for a gold standard annotation pipeline.

Table 7. Summary of features evaluated between genome annotation tools

Feature EcoCyc PGAP Prokka AMG*
CDs 4357 4377 4305 4325
gene 4566 4500 4416 4325
misc_feature 48 0 0 0
misc_recomb 1 0 0 0
mobile_element 49 0 0 0
ncRNA 72 13 0 0
rRNA 22 22 22 22
regulatory 0 0 0 0
rep_origin 1 0 0 0
repeat_region 697 2 0 0
source 1 1 1 1
tRNA 86 87 88 86
tmRNA 0 1 1 0

*AMGP: Advanced Microbial Genome Annotation pipeline developed by ATCC


As life science research progresses, the quality of data becomes increasingly important. Yet, the whole-genome sequencing data available in various public databases are frequently incomplete, fragmented, and contain errors. This is problematic as comprehensive, high-quality sequence data are essential for making correlations between in silico analyses and for translating research into clinical diagnostics and other regulated applications.

Therefore, as part of our initiative to enhance the authentication of biological materials, we have developed a standardized genome sequencing and assembly workflow to provide researchers with reference-grade genomes that are matched to authenticated ATCC strains. Our optimized methodology uses a hybrid assembly approach that combines the power of highly accurate Illumina short reads with the revolutionary scaffolding ability of Oxford Nanopore ultra-long reads. We have then taken our workflow one step further by accompanying each stage of the process with rigorous quality control analyses that ensure our data is the highest quality possible.

To date, ATCC’s ongoing genome sequencing efforts have produced over 250 authenticated reference-quality genomes for bacterial species pertinent to human health, diagnostics, quality control, microbiome studies, and antimicrobial resistance and rediscovery applications. We provide these sequences in a cloud-based portal that will enable researchers to quickly find and compare the data they need. We believe that this robust and high-quality ATCC genomic database will be of immense use to researchers for the development, verification, and validation of NGS-based assays in diverse areas of microbiology.

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We would like to thank Nick Greenfield, MA, and Team at One Codex for their expertise and support with establishing the ATCC Genome Portal. Additionally, we thank Dev Mittar, PhD, for his innovation and leadership as well as Sam Minot, PhD, for his insight and helpful suggestions throughout the design and development process.