SU-DHL-8 (ATCC® CRL-2961)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: lymph node; derived from metastatic site: peritoneal effusion  /  Disease: Large cell lymphoma

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Organism Homo sapiens, human
Tissue lymph node; derived from metastatic site: peritoneal effusion
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast-like
Culture Properties suspension, grow as single cells and small clusters
Biosafety Level 1
Disease Large cell lymphoma
Age 59 years
Gender Male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images CRL-2961 Micrograph
Clinical Data 59 years
Caucasian
Male
Receptor Expression Fc (not expressed), IgMEAC (not expressed), IgGEA (not expressed), E (not expressed)
Genes Expressed Ig +; IgM-, IgG-, IgA-, IgD-, Lambda-, Kappa-
Comments

The depositor states that this cell line is negative for Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
This cell line is negative for Canadida ingestion.
This is a null cell line (absence of all known markers) based on the classification of diffuse histiocytic lymphomas.
These cells exhibit no response to LY294002-induced apoptosis.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. Subculture the cells when cell density is ~ 1.0 x 106 to 2.5 x 106 cells/mL for higher viability. An inoculum of 1.0 x 105 to 4.0 x 105 cells/mL is recommended.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended.
Medium renewal: Every 2 to 4 days (depending on cell density)
Cryopreservation Storage temperature: liquid nitrogen vapor phase
Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 11, 13
D13S317: 11, 13
D7S820: 8
D16S539: 12
CSF1PO: 11, 12
vWA: 15, 19
THO1: 6, 9
TPOX: 8
Amelogenin: X
Year of Origin 1978
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220