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NCI-H1355 [H1355]


NCI-H1355 [H1355] is a cell line that was isolated from the lungs of a 53-year-old, White male with stage 4 adenocarcinoma. This product can be used for cancer research.
Product category
Human cells
Homo sapiens, human
Adenocarcinoma; Stage 4
3D cell culture
Product format
Storage conditions
Vapor phase of liquid nitrogen
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Growth properties
The cell line was established in March 1986 from pleural effusion metastasis of lung.
53 years
modal number = 54; range = 43-66
Pleural effusion

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.
  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium

The base medium for this cell line is DMEM:F12 (ATCC 30-2006).To make the complete growth medium, add the following components to 475 mL of the base medium: 25 mL ACL-4 20X Cocktail (see below), 7 mL Bovine Serum Albumin (Sigma A7979); Transferrin, 1 mg/mL stock- use 10 µL/1 mL culture medium  (see preparation notes below); EGF, 1 µg/mL stock (see preparation notes below)- use 1 µL/1 mL culture medium.

20 X ACL-4 cocktail is made up of 

  • 24 mL RPMI 
  • 40 mL of 4mg/mL Human Insulin
  • 40 mL of 5 µM Sodium Selenite 
  • 8 mL 10 mM Ethanolamine
  • 8 mL of 10 mM Phosphorylethanolamine
  • 7 uL 30 µM Triiodo-L-thyronine
  • 40 mL of 100 mM Sodium Pyruvate
  • 80 mL 1 M HEPES
  • 80 mL 200 mM L-glutamine
  • 80 mL 5 µM Hydrocortisone

To prepare a 1mg/mL Transferrin stock solution, aseptically combine:

  • 1 vial of Transferrin containing 100 mg (sterile lyophilized)  (Sigma T-1147)
  • 100 mL serum free medium EMEM (ATCC 30-2003) or RPMI-1640 (ATCC 30-2001)

Sterile filter using 0.22 µm filter

Note: Transferrin is stable at 2-8°C for up to 7 days. Do not use Transferrin which has been at 2-8°C for more than 7 days.

To prepare a 1 µg/mL EGF stock solution, aseptically combine:

  • 1 vial of 10 µg Gibco™ EGF (ThermoFisher Scientific PHG0314)
  • 10 mL PBS with 0.1% BSA (150 mL Ca/Mg free PBS + 0.15 g BSA) or equivalent

To prepare 0.1% BSA solution

  • 150 mL Ca/Mg free PBS (ATCC 30-2200)
  • 0.15 g BSA (Sigma A8022)

Note: Aliquot in working volumes and store at -20°C. Avoid freeze/thaw cycles. EGF is stable for 1 year after reconstitution when prepared and stored as indicated above.

Note: Once prepared, the above ACL-4 basal media is stable when stored at 2-8°C for up to 1 month.

Because of limited stability, the following components should be added to an aliquot of the above culture medium fresh prior to seeding or performing fluid additions. Complete medium with the below components can be stored at 2.0 to 8.0°C and expires in 7 days.

95% Air, 5% CO2
Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

  1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).
  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
  3. Transfer the vial contents to a centrifuge tube containing   9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.
  4. Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm2 culture flask.  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
  5. Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
Subculturing procedure
Cultures can be maintained by the addition of fresh medium or by replacement of medium. Alternatively, cultures can be established by centrifugation and subsequent resuspension of the pellet. The cells grow in floating aggregates of round cell clusters.
Medium Renewal: twice weekly
Note: Add fresh medium as the cell density increases.
Reagents for cryopreservation
Complete growth medium supplemented with 10% (v/v) FBS and 5% (v/v) DMSO (ATCC 4-X)

Quality control specifications

Mycoplasma contamination
Not detected
STR profiling
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 11,13
D7S820: 12
TH01: 9
TPOX: 9,12
vWA: 14,17
D3S1358: 18
D21S11: 31,32.2
D18S51: 11,17
Penta_E: 14,19
Penta_D: 13,15
D8S1179: 14,16
FGA: 22,23
D19S433: 14
D2S1338: 21,23


Deposited as
Homo sapiens
AF Gazdar, JD Minna
Year of origin
Special collection
NCRR Contract

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

This cell line was deposited by Dr. A. Gazdar and is provided for research purposes only. This material is subject to the following restrictions in addition to those outlined in the ATCC Material Transfer Agreement:

  1. Transfers - Biological Materials may not be transferred to third parties for purposes of sale, or producing for sale
  2. Commercial Use - all for-profit and non-profit Recipients must obtain a commercial use license prior to Commercial Use
Any proposed Commercial Use with these cells must first be negotiated with:

National Cancer Institute (NCI)
Email: [email protected]


Frequently Asked Questions


Curated Citations

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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