454C11
CRL-3774 ™
This material is ATCC manufactured progeny of 454C11 cited in US Pat. No. 4,753,894 as HB-8484.
- Product category
-
Animal cells
- Product type
-
Hybridoma
- Organism
-
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
- Cell type
- hybridoma: b lymphocyte
- Morphology
- lymphoblast
- Disease
-
Cancer
- Applications
-
Immunology
- Product format
- Frozen
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Specific applications
-
The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue.By immunofluorescence, the antibody reacts with epithelia of several normal and neoplastic tissues.
This material is ATCC manufactured progeny of 454C11 cited in US Pat. No. 4,753,894 as HB-8484.
Characteristics
- Growth properties
- Suspension
- Tumorigenic
- Yes; Yes, forms ascites in BALB/c mice
- Genes expressed
- immunoglobulin, monoclonal antibody, against human breast cancer cells
- Isotype
-
IgG2a
- Comments
-
Animals were immunized either with live cells or with membrane extracts from human breast cancer cell lines.Spleen cells were fused with Sp2/0-Ag14 myeloma cells.The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue.By immunofluorescence, the antibody reacts with epithelia of several normal and neoplastic tissues.
Handling information
- Complete medium
- Dulbecco's modified Eagle's medium, with HAT 80%; fetal bovine serum, 20%
- Handling procedure
-
Part A. FROZEN CELLS
Vol./Ampule: 1.0 mL.
Recommended Handling Upon Receipt: Initiate culture as soon as possible upon receipt. Thaw by rapid agitation in 37°C water bath. See instructions on back.
Dilute ampule contents with culture medium (see batch data above). Add fresh
medium (depending on cell density) every 2-3 days. - Subculturing procedure
-
Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Quality control specifications
- Mycoplasma contamination
- Not detected
History
- Deposited as
- This material is ATCC accessioned progeny of 454C11 cited in US Pat. No. 4,753,894 as HB-8484.
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Frequently Asked Questions
References
Curated Citations
Ring DB, et al. Monoclonal anti-human breast cancer antibodies. US Patent 4,753,894 dated Jun 28 1988
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