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Pluripotent Stem Cell SFM XF/FF (ATCC® ACS-3002)

Permits and Restrictions

View Permits

Product Format frozen 500 mL
Storage Conditions Complete medium is stored at -20°C
This ready to use, complete medium, is tested for pH, osmolality, endotoxins and sterility. Each lot of Pluripotent Stem Cell SFM XF/FF is tested functionally with human stem cells to ensure that pluripotency is maintained as determined by expression of pluripotent markers.
Biosafety Level N/A
Quality Control Specifications

Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also tested for the ability to support the growth of cell lines using both sequential subcultures and plating efficiencies. pH, osmolality, and endotoxin tests are also performed as indicated below.




6.70 to 7.30


280 to 320

mOsm/kg H2O

Sterility Testing



≤ 1.0 EU/mL

Functional Stem Cell Culture

Maintains pluripotency on hESC as determined by expression of pluripotent marker

*Please consult the Certificate of Analysis for lot-specific test results.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Antibiotics in Pluripotent Stem Cell SFM XF/FF (ATCC ACS-3002)

    Date Updated: 10/30/2014

  2. Filtering Pluripotent Stem Cell SFM XF/FF (ATCC ACS-3002) media

    Date Updated: 10/30/2014

  3. iPSC - bFGF to pluripotent stem cell medium formulations

    Date Updated: 3/27/2014

  4. iPSC - ATCC Pluripotent stem cell media vs. FBS-containing media

    Date Updated: 1/23/2014

  5. Pluripotent Stem Cell SFM XF/FF Basal Medium Components

    Date Updated: 2/3/2014


Bergqvist C, et al. An inner nuclear membrane protein induces rapid differentiation of human induced pluripotent stem cells. Stem Cell Res 23:33-38, 2017. PubMed: 28668644