Mouse embryonic stem cells (mESCs) were first isolated and propagated in culture in 1981. mESCs are typically isolated from blastocysts from the inner cell mass of 3.5-day-old embryos and have the potential to generate every cell type found in the body. They can be maintained in an undifferentiated, pluripotent state by culture with leukemia inhibitory factor (LIF) on a feeder layer of mitotically arrested mouse embryonic fibroblasts or in a feeder-free environment using gelatin-coated flasks.
mESCs are invaluable in understanding many aspects of cell and developmental biology, including cell cycle regulation, cellular interactions during development, and the control of gene expression. mESCs will continue to be an important system for understanding stem cell and tissue biology and for creating transgenic or knock-out mice, used as in vivo disease models, by homologous recombination. Recent applications of mESCs include identifying factors that maintain pluripotency, human genetic disease research, identifying novel differentiation factors, and for cancer biology.
ATCC provides a large collection of the most influential and widely cited mouse ES cells.
Mouse embryonic stem cells resources
Stem cell culture guide
Stem cells hold great promise for biomedical research. Even with the knowledge that scientists have gained over the past decade, stem cell culture remains challenging. Different types of stem cells often require different culture conditions. Learn how you can successfully cultivate mESCs.Get Expert Tips
STR Profiling for Mouse Cell Lines: A New Tool to Combat Cell Line Misidentification
Short tandem repeat profiling (STR) has addressed authentication issues in human cell lines, but there has not been a validated method for identifying mouse cell lines beyond the species level, until now. In this presentation, we describe ATCC’s STR profiling services, focusing on a new mouse STR profiling method.Explore Mouse STR