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Primary Cells

Red and blue phalloidin human umbilical vein endothelial cells.

Essential tools to advance your life science research and drug discovery

Primary cell cultures more closely mimic the physiological state of cells in vivo and generate the most biologically relevant data. Primary cultures are cells freshly isolated from organ tissue and maintained for growth in vitro. Each mammalian tissue type is derived from the embryonic germ layer consisting of ectoderm, endoderm, and mesoderm, which differentiate into the many cell types that organize into tertiary structures such as skin, muscle, blood and blood vessels, internal organs, bone and cartilage, and components of the nervous system. Epithelial cells, fibroblasts, keratinocytes, melanocytes, endothelial cells, muscle cells, hematopoietic cells, and mesenchymal stem cells are the cell types most frequently used in research in primary cell culture.

Benefits of primary cells

Human primary cells are commonly used in vitro for preclinical and investigative biological research, such as studies of intercellular and intracellular communication, developmental biology, and the mechanisms seen in cancer, Parkinson’s disease, and diabetes. Historically, investigators employed immortalized cell lines in tissue function; however, the use of cell lines containing gross mutations and chromosomal abnormalities may be poor indicators of normal cell phenotype and progression of early-stage disease. Human primary cells derived from particular tissues and maintained with the appropriate primary cell culture media and supplements now represent a more advanced model for recapitulating that tissue cell type than immortalized cells.

Human primary cells by tissue type

Blue and green high resolution fibroblast cells.

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Human primary cells resources

Cluster of round, green, textured, neutrophil-ingesting  Methicillin-resistant Staphylococcus aureus.

In Vitro Differentiation of Macrophages and Dendritic Cells from Primary Human CD14+ Monocytes

This study demonstrates the differentiation potential of cryopreserved primary human CD14+ monocytes towards macrophage and dendritic cell lineages for applications such as cancer immunology, vaccine development, inflammation, bone disease, immunosuppression, disease resolution, and tissue repair studies.

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Small, round, purple and green uterine smooth muscle cells.

Primary Cells for Drug Screening

We explored applications of human primary cells for cytotoxicity assays and drug screening. The results demonstrate that various types of human primary cells can be used for preclinical applications including drug screening, toxicology, and modeling physiological responses.

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Learn why hTERT-immortalized primary cells are the best of both worlds

Primary Cells

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