Primary human hepatocytes are considered the gold standard for in vitro liver models due to their high predictive value in drug metabolism and toxicity studies. They can provide early insights into how a drug will behave in the human body, potentially reducing the risk of adverse effects in clinical trials.  ATCC hepatocytes are meticulously isolated and characterized to ensure the highest quality and performance for your drug development needs.

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• Premium plate-ability: HepatoXcell™ Pro 7-day plateable hepatocytes offer extended viability and functionality, enabling longer-term studies of liver metabolism, toxicity, and disease modeling. Their stable phenotype supports repeated dosing and chronic exposure assays, making them ideal for ADME-Tox applications and high-content screening in drug development workflows.
• Comprehensive characterization: Each batch undergoes rigorous testing to ensure consistency and reliability, including assessments of enzyme activity, gene expression, drug-drug interaction assay and metabolic function.
• Batch-specific DMPK data available: Easily access downloadable Certificates of Analysis (COAs) for each batch, featuring key DMPK metrics such as metabolite profiling, induction assays and transporter activity, supporting informed selection and regulatory documentation.
• Your success, supported by our expertise: Accelerate your research with confidence. Our dedicated technical team offers expert consultation, training videos, and on-site support to help you optimize assay development and troubleshoot challenges, ensuring you get the most out of your hepatocyte models from day one.
• Easy lot selection: Use our lot selection tool to easily select your lot of HepatoXcell™ based on donor demographics, gene expression, enzyme activities, and other parameters, at the click of a mouse.
• Detailed culture protocols: To maximize the viability and functionality of our primary human hepatocytes, we provide detailed thawing and plating, media preparation, and maintenance and feeding protocols tailored to your research needs.
• Global shipping: We offer reliable shipping options worldwide, ensuring timely delivery to your laboratory. HepatoXcell™ hepatocytes are shipped in temperature-monitored liquid nitrogen tanks to maintain ultra-low temperatures. Upon receipt, store immediately or thaw and begin your assay.

HepatoXcell™ Pro cells maintain stable hepatic function and morphology in sandwich culture. (A) Phase-contrast and (B) confocal images (7 days culture) show polarized distribution of hepatocytes and the formation of hepatic structures such as canalicular membranes in sandwich culture.

Primary human hepatocytes are invaluable for studying human liver biology, enabling accurate modeling of drug metabolism, toxicity, genetic diseases, and therapeutic responses in both research and clinical applications.

• Drug metabolism and pharmacokinetics (DMPK)
• Toxicology and safety assessment
• Liver disease modeling
• Regenerative medicine and cell therapy
• Transporter studies
• Microphysiological systems development

HepatoXcell™ Pro Primary Human Hepatocytes thrive in a variety of culture systems. Using a standardized protocol, we cultured cells in two microfabricated liver-chip designs: a flat-bed design and a 3-D meshed-bed design. This study demonstrates the quality of HepatoXcell across two distinct liver-chip platforms, highlighting their applicability in microfluidic systems. These data supports HepatoXcell’s use in pharmacological research, including drug discovery, toxicity testing, and disease modeling.