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HepatoXcell™ Eco: Primary Human Hepatocytes

PCS-450-012

HepatoXcell™ logo

HepatoXcell™ Eco are Primary Human Hepatocytes Suspension Cells, derived from normal, healthy, human liver tissues.

Product category
Human cells
Product type
Primary cell
Organism
Homo sapiens, human
Morphology
round and often in clusters while in suspension
Tissue
Liver
Applications
Toxicology
Cancer research
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
In vitro ADME assays

Characteristics

Cells per vial
≥ 4.0 x 106
Growth properties
Suspension

Handling information

Complete medium
The complete media used is HepatoXcell™ Primary Hepatocyte Maintenance Medium 1x (ATCC PCS-450-034).
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure

Unpacking and storage instructions

• Check all containers for leakage or breakage.
• Remove the frozen cells from the liquid nitrogen dry shipper and immediately place the cells at a nitrogen vapor dewar with a temperature below -130°C, until ready for use.

Required media and supplement
• One bottle of each of the following: Hepatocyte Thaw Media (ATCC PCS-450-032), Hepatocyte Maintenance Media (ATCC PCS-450-034).

Handling procedure
1. Refer to the batch specific information for the total number of viable cells recovered post-thaw for any lot of PCS-450-012.
2. Add 19 mL of pre-warmed Hepatocyte Thaw Media to a 50 mL centrifuge tube.
3. Remove one vial of PCS-450-012 from storage and thaw the cells by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1 to 2 minutes).
4. Remove the vial from the water bath as soon as the contents are thawed leaving a small ice pellet and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point onward should be carried out under strict aseptic conditions.
5. Gently pour the contents of the vial into the 50 mL centrifuge tube.
6. Using a wide bore pipette tip wash the vial with 1 mL of the Hepatocyte Thaw Media suspension to retrieve any cells left in the vial.
Note: When pipetting Hepatocytes NEVER pipette up and down to mix, instead gently rock or shake the tube.
7. Centrifuge at 100 x g for 10 minutes.
8. Carefully remove the supernatant and resuspend with 1 mL of prewarmed Hepatocyte Maintenance Media.
9. Gently rock or shake the tube to resuspend the cells, then add an additional 1 mL of Hepatocyte Maintenance Media.
10. Mix gently by rocking or shaking to ensure a homogenous suspension.
11. Perform a cell count using the Trypan Blue Exclusion Method.
12. Using the total number of viable cells, dilute the cells to 1,000,000 cells/mL.
13. Perform assays taking the appropriate number of cells as suitable to the workflow.

Quality control specifications

Mycoplasma contamination
Not detected
Virus testing
Hepatitis B virus (HBV): Not detected
Hepatitis C virus (HCV): Not detected
Human Immunodeficiency virus (HIV): Not detected

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Frequently Asked Questions

References

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