A6
CCL-102 ™
A6 is an animal cell line exhibiting epithelial morphology that was isolated from the kidney tissue of a South African clawed frog.
- Product category
-
Animal cells
- Organism
-
Xenopus laevis, frog, South African clawed
- Morphology
- epithelial
- Tissue
- kidney
- Disease
-
Normal
- Applications
-
3D cell culture
- Product format
- Frozen
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
Characteristics
- Growth properties
- Adherent
- Age
- adult
- Gender
- Male
Handling information
- Unpacking and storage instructions
-
- Check all containers for leakage or breakage.
- Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
- Complete medium
- NCTC 109 medium, 75%; distilled water, 15%; fetal bovine serum, 10%
- Temperature
-
26°C
- Handling procedure
-
The flask was seeded with cells (see specific batch information above for concentration), grown and completely filled with medium to prevent loss of cells in transit. Remove all of the medium (which can be saved and used as fresh medium) except for a sufficient volume (5-10 ml) to cover the floor of the flask. Incubate at 26°C. The shipping medium contains reduced sodium bicarbonate suitable for an atmosphere without CO2. NCTC 109 usually contains 2.2 grams of sodium bicarbonate per liter and should be incubated in a 5% CO2 in air incubator. Sometimes in transit the cultures are handled roughly and most of the cells become detached and float in the culture medium. If this has occurred remove the entire contents ofthe flask and centrifuge at 300 x g for 15 minutes. Draw off the excess supernatant medium, resuspend the cells in 10 ml of the culture medium and plant the entire cell suspension in a single flask of suitable size (about 25 sq. cm.).
- Subculturing procedure
-
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommendedMedium Renewal: Twice per weekRemove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
- Reagents for cryopreservation
- Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)
Quality control specifications
- Mycoplasma contamination
- Not detected
History
- Deposited as
- Xenopus laevis
- Depositors
- KA Rafferty
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969.
Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803
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