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MMA (ATCC® HB-78)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with human adherent mononuclear cells and granulocytes.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human monocyte
Cellular Products
immunoglobulin; monoclonal antibody; against human monocyte
Comments
Animals were immunized with the U-937 histiocytic cell line (ATCC CRL-1593).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with human adherent mononuclear cells and granulocytes.
The antibody does not react with circulating T cells, B cells, erythrocytes or platelets.
The antibody is cytotoxic.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM; kappa light chain
Name of Depositor JF Kearney, H Kubagawa
Deposited As mouse (B cell); mouse (myeloma)
References

Hanjan SN, et al. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin. Immunol. Immunopathol. 23: 172-188, 1982. PubMed: 6179666

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hanjan SN, et al. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin. Immunol. Immunopathol. 23: 172-188, 1982. PubMed: 6179666