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UC8-1B9 (ATCC® CRL-1968)

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is useful as a control monoclonal antibody when using other hamster derived hybridomas.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is useful as a control monoclonal antibody when using other hamster derived hybridomas.
Genes Expressed
immunoglobulin; monoclonal antibody; against dinitrophenyl (DNP)
Cellular Products
immunoglobulin; monoclonal antibody; against dinitrophenyl (DNP)
Comments
Animals were immunized with DNP derivatized KLH (keyhole limpet hemocyanin).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is useful as a control monoclonal antibody when using other hamster derived hybridomas.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG
Name of Depositor JA Bluestone
Deposited As hamster, Armenian (B cell); mouse (myeloma)
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation