4D9D4 (ATCC® CRL-1818)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with Drosophila invected protein produced in E. coli.
The antibody cross-reacts with engrailed protein of Drosophila, grasshopper, leech, chicken, zebra fish, lobster and crayfish.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with Drosophila invected protein produced in E. coli.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the invected and engrailed proteins of Drosophila melanogaster
Cellular Products
immunoglobulin; monoclonal antibody; against the invected and engrailed proteins of Drosophila melanogaster
Comments
Animals were immunized with Drosophila invected protein produced in E. coli.
Spleen cells were fused with NS-1 myeloma cells.
The antibody cross-reacts with engrailed protein of Drosophila, grasshopper, leech, chicken, zebra fish, lobster and crayfish.
It does not recognize mouse (En-1) or sea urchin engrailed proteins.
The epitope recognized by the antibody is within the homeodomain.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor TB Kornberg
Deposited As mouse (B cell); mouse (myeloma)
References

Patel NH, et al. Expression of engrailed proteins in arthropods, annelids, and chordates. Cell 58: 955-968, 1989. PubMed: 2570637

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Patel NH, et al. Expression of engrailed proteins in arthropods, annelids, and chordates. Cell 58: 955-968, 1989. PubMed: 2570637