IMR-32 (ATCC® CCL-127)

Organism: Homo sapiens, human  /  Cell Type: neuroblast  /  Tissue: brain; derived from metastatic site: abdominal mass  /  Disease: neuroblastoma

Organism Homo sapiens, human
Tissue brain; derived from metastatic site: abdominal mass
Cell Type neuroblast
Product Format frozen
Morphology fibroblast; neuroblast
Culture Properties adherent
Biosafety Level 1
Disease neuroblastoma
Age 13 months
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype Stable male karyotype with stemline number of 49. Two large marker chromosomes with submedian centromeres. A deletion in one number 1 chromosome: One number 16 chromosome missing; two extra chromosomes in C group. Sublines with 50 and 48 chromosomes differ from those with 49 chromosomes by having an extra or missing C group chromosome respectively. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Images
Derivation
The IMR-32 cell line was established by W.W. Nichols, J. Lee and S. Dwight in April, 1967 from an abdominal mass occurring in a 13-month-old Caucasian male. The tumor was diagnosed as a neuroblastoma with rare areas of organoid differentiation.
Clinical Data
13 months
Caucasian
male
Virus Susceptibility Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Herpes simplex virus
Vaccinia virus
Human Coxsackievirus B3
Human poliovirus 3
Virus Resistance {8B5AD8F2-1676-4399-B5A1-85F54726A74D}
Comments
Two cell types are present. Predominant is a small neuroblast-like cell.The other is a large hyaline fibroblast.

IMR-32 cells may pile up and grow in patches. 

IMR-32 cells may not become 100% confluent.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels. Maintain cultures at a cell concentration between 4x104 and 4 x 105 cells/cm2.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9
D16S539: 8
D5S818: 11,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 11
vWA: 15
Isoenzymes
G6PD, B
Population Doubling Time approximately 20 hrs.
Name of Depositor WW Nichols
Passage History
The cell line was submitted to the American Type Culture Collection in the 36th passage. It has been demonstrated that the cells can be propagated successfully beyond the 80th serial subculture.
Year of Origin April, 1967
References

Tumilowicz JJ, et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 30: 2110-2118, 1970. PubMed: 5459762

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614

Basic Documentation
Other Documentation
References

Tumilowicz JJ, et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 30: 2110-2118, 1970. PubMed: 5459762

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614