ATCC - Resources for Cell Biology

Application Note No. 1: 3T3-L3 Cells — 3T3-L1 cells lose contact inhibition and adipogenic potential when maintained in cult ure at confluence. (972KB/.pdf)

Application Note No. 2: Tips for successful sub-culturing: Dissociation Method — Tailoring your dissociation method to your cell line. (523KB/.pdf)

Application Note No. 4: CellMatrix Basement Membrane Gel — CellMatrix Basement Membrane Gel supports in vitro angiogenesis assays. (980KB/.pdf)

Application Note No. 6: Universal Mycoplasma Detection Kit — check your cultures for mycoplasma contamination. (972KB/.pdf)

Application Note No. 8: Telomerase-immortalized microvascular endothelial (TIME) Cells — Growth rate and endothelial character are better maintained in ATCC^® VCBM -VEGF medium. (968KB/.pdf)

Application Note No. 14: Human Bronchial/Tracheal Epithelial Cells — Improving Functional Studies. (530KB/.pdf)

Application Note No. 16: Differentiation and expansion of hematopoietic precursor cells from bone-marrow derived CD34+ progenitors — (512KB/.pdf)

Application Note No. 17: htert-immortalized and Primary Keratinocytes Differentiate into Epidermal Structures in 3d Organotypic Culture (500KB/.pdf)

Application Note No. 19: In vitro angiogenesis assay using the ATCC^® Angio-Ready™ System (4.11MB/.pdf)

Application Note No. 21: In vitro differentiation of macrophages and dendritic cells from primary human CD14+ monocytes (510KB/.pdf)

Application Note No. 23: Comprehensive Gene Expression Analysis and Neurotoxicity Testing of Human iPSC-Derived Neural Progenitor Cells and Neurons (2.8MB/.pdf)

Application Note No. 24: Establishment and Characterization of a Kidney-Drug Interaction Model by Stably Expressing hOAT1 in HEK 293T/17 Cells (732KB/.pdf)

Application Note No. 25: The Generation of an EML4-ALK Fusion NSCLC Isogenic Cell Line Relevant for Drug Discovery and Development (432KB/.pdf)

Application Note No. 26: Directed differentiation of gastrointestinal epithelial organoids using ATCC^® CellMatrix Basement Membrane from multiple human ATCC iPSC lines (748 KB/.pdf)

Application Note No. 31: A simple and rapid alternative to ⁵¹chromium or fluorescent dye loading for quantification of natural killer cell activity: ATCC^® K-562-GFP™ cells (322KB/.pdf)

Application Note No. 32: Genetically Modified Renal Proximal Tubule Epithelial Cells (3.63MB/.pdf)

Application Note No. 33: Protocol for the thawing, expansion, and cryopreservation of mouse small intestinal organoids (2.37MB/.pdf)

ATCC Excellence in Research Recorded Webinars — Each webinar features highly experienced Scientists who demonstrates how to utilize ATCC products and services to advance your research!

The Scientist — Sharing Made Easy

GEN — Safeguarding Biological Material

Drug Discovery News — Working together to eradicate cell line misidentification and contamination: New practices and policy

How to Revive Cultures

Technical Bulletin No. 3: Cryogenic Preservation of Animal Cells (50.2KB/.pdf)

Technical Bulletin No. 4: Guide to Subculturing Cell Line Monolayers (47.8KB/.pdf)

Technical Bulletin No. 7: Passage Number Effects In Cell Lines (220KB/.pdf)

Technical Bulletin No. 8: Cell Line Authentication Test Recommendations (483KB/.pdf)

Technical Bulletin No. 9: Chemically-induced Differentiation of ATCC^® CL-173™ (3T3-L1) Using Single Component Commercially Available Reagents (61KB/.pdf)

Technical Bulletin No. 10: Lymphocyte Transformation Using ATCC^® VR-1492™ (241KB/.pdf)

Cell Authentication — STR Essentials

Cell Lines Misidentification: the beginning of the end

Cryogenic Preservation of Animal Cells (50.2KB/.pdf)

Guide to Subculturing Cell Line Monolayers (47.8KB/.pdf)

Guidelines for Testing and Publishing (47.8KB/.pdf)

Misidentified Cell lines

Mycoplasma Detection (585 KB/.pdf)

Passage Number Effects In Cell lines (377KB/.pdf)

STR Profiling (471 KB/.pdf)

The Costs of Using Unauthenticated, Over-Passaged Cells (391 KB/.pdf)

Titered Mycoplasma Strains Panel (906KB/.pdf)