Resources for Cell Biology

Cell Culture Guides

• Animal Cell Culture Guide — tips and techniques for continuous cell lines (1.07 MB/.pdf)
• Breast Cancer Resource Book
• hTERT Immortalized Cell Culture Guide — tips and techniques for culturing hTERT immortalized cells (1.06 MB/.pdf)
• Stem Cell Culture Guide — tips and techniques for culturing stem cells (2.36 MB/.pdf)
• Primary Cell Culture Guide — tips and techniques for culturing primary cells (784 KB/.pdf)
• Cryopreservation Technical Manual (404KB/.pdf)

Application Notes

• 3T3-L3 Cells — 3T3-L1 cells lose contact inhibition and adipogenic potential when maintained in cult ure at confluence. (972KB/.pdf)
• CellMatrix Basement Membrane Gel — CellMatrix Basement Membrane Gel supports in vitro angiogenesis assays. (980KB/.pdf)
• HEKPlus Protein Expression System — easy-to-use mammalian protein expression system. (964KB/.pdf)
• Telomerase-immortalized microvascular endothelial (TIME) Cells — Growth rate and endothelial character are better maintained in ATCC^® VCBM -VEGF medium. (968KB/.pdf)
• Tips for successful sub-culturing: Dissociation Method — Tailoring your dissociation method to your cell line. (523KB/.pdf)
• Universal Mycoplasma Detection Kit — check your cultures for mycoplasma contamination (972KB/.pdf)

Online Video Resources

• ATCC Excellence in Research Upcoming Webinars — Each webinar features highly experienced Scientists who demonstrates how to utilize ATCC products and services to advance your research
• ATCC Excellence in Research Recorded Webinars 
• CORNING® Webinars — Free online cell culture and assay training

Published Articles

• The Scientist — Sharing Made Easy
• GEN —  Safeguarding Biological Material
• Drug Discovery News — Working together to eradicate cell line misidentification and contamination: New practices and policy

Technical Bulletins and Culture Tips

• How to Revive Cultures
• CORNING®   Technical Articles and Protocols
• Technical Bulletin No. 3: Cryogenic Preservation of Animal Cells (50.2KB/.pdf)
• Technical Bulletin No. 4: Guide to Subculturing Cell Line Monolayers (47.8KB/.pdf)
• Technical Bulletin No. 7: Passage Number Effects In Cell Lines (220KB/.pdf)
• Technical Bulletin No. 8: Cell Line Authentication Test Recommendations (483KB/.pdf)
• Technical Bulletin No. 9: Chemically-induced Differentiation of ATCC^® CL-173™ (3T3-L1) Using Single Component Commercially Available Reagents  ((61KB/.pdf)
• Technical Bulletin No. 10: Lymphocyte Transformation Using ATCC^® VR-1492™ (241KB/.pdf)

Cell Authentication

• Cell Line Authentication Test Recommendations (483KB/.pdf)
• STR Profiling (471 KB/.pdf)
• Mycoplasma Detection (585 KB/.pdf)
• Titered Mycoplasma Strains Panel  (906KB/.pdf)
• Cell Lines Misidentification: the beginning of the end
• Maintaining High Standards in Cell Culture (1.49MB/.pdf)
• The Costs of Using Unauthenticated, Over-Passaged Cells (391 KB/.pdf)
• Guide to Subculturing Cell Line Monolayers (47.8KB/.pdf)
• Guidelines for Testing and Publishing (47.8KB/.pdf)
• Cryogenic Preservation of Animal Cells (50.2KB/.pdf)
• Passage Number Effects In Cell lines (377KB/.pdf)
• Misidentified Cell lines

Cell Biology Brochures

• Cell Lines by Gene Mutation Brochure (1.68 MB/.pdf)
• Brain Tumor Cell Lines (657 KB/.pdf)
• Breast and Gynecological Tumor Cell Lines (335 KB/.pdf)
• Certified Reference Material (300 KB/.pdf)
  
• Gastrointestinal Tumor Cell Lines (344 KB/.pdf)
• Genetic Alteration Panels Brochure (1.8 MB/.pdf)
  
• Genitourinary Tumor Cell Lines (783 KB/.pdf)
• Genomic DNA from Human Cell Lines (256 KB/.pdf)
  
• hTERT Immortalized Cell Lines (263 KB/.pdf)
  
• Lung Tumor Cell Lines (808 KB/.pdf)
• p53 Hotspot Mutation Cell Panels Brochure (1.85 MB/.pdf)
  
• Primary Cell Solutions Brochure (1.42 MB/.pdf)
  
• Primary Cell Solutions Collection (686 KB/.pdf)
  
• Stem Cell Solutions Brochure (279KB/.pdf)
  
• Tumor/Normal Matched Cell Line Pairs (362 KB/.pdf)
• Tumor Cell Panels Brochure (2 MB/.pdf)

Cell Culture Media and Kits

• ATCC High-Performance Media and Sera Brochure (739 kb/.pdf)
• Comparison of the MTT and XTT Cell Proliferation Assay Kits (561KB/.pdf)
• Primary Cell Culture Media, Growth Kits and Reagents
• Stem Cell Culture Reagents and Media
• Transfex™ (332 KB/.pdf)

References and Additional Publications

• Selected References Regarding Cell Culturing (662KB/.pdf)
• Newsletters 

Quick Links

• FAQs 

ATCC technical documents and brochures can be downloaded using Adobe Acrobat Reader

While ATCC uses reasonable efforts to include accurate and up-to-date information in this technical help section, we make no warranties or representations as to its accuracy. ATCC is not responsible for any errors or omissions or for the results obtained from the use of this information. Content of the site is subject to change without notice.