Cell Biology Resources

A compilation of cell biology related application notes, technical guides, bulletins, videos, and more - available for downloading and viewing.

Animal Cell Culture Guide

Animal Cell Culture Guide
Primary Cell Culture Guide

Primary Cell Culture Guide
Stem Cell Culture Guide

Stem Cell Culture Guide

Resource Pages

A549 Cells and Derivatives

Angiogenesis Resources

Cardiovascular Research

Cancer Resources

Cancer and Normal Cell Lines by Tissue Type

Cancer Immunology Research Tools

Cell Authentication Matters

Cell Collections, Cell Culture Systems, and Cell Health Detection Kits

Cell Line DNA

CRISPR ToolBox Cells

Differentiated iPSCs

Drug Discovery

Enteric Disease Research Materials

Epithelial-mesenchymal Transition

Exosomes

Immunological Research Tools

iPSC Research Resources

Isogenic Cell Lines

Luciferase Cells

Mouse STR Profiling

Neurobiology Research Tools

Primary Cell Selection Guide

Respiratory Disease

Screening Tools

STR Profiling Analysis

Toxicology Tools

Transfection Tools

Women’s Health Research Tools

Your Trusted Source

Cell Culture Guides

Animal Cell Culture Guide — tips and techniques for continuous cell lines (1.07 MB/.pdf)

Cryopreservation Technical Manual (404KB/.pdf)

hTERT Immortalized Cell Culture Guide — tips and techniques for culturing hTERT immortalized cells (1.06 MB/.pdf)

Primary Cell Culture Guide — tips and techniques for culturing primary cells (784 KB/.pdf)

Stem Cell Culture Guide — tips and techniques for culturing stem cells (2.36 MB/.pdf)

Brochures

Angiogenesis Resources (972KB/.pdf)

ATCC High-Performance Media and Sera Brochure (739 kb/.pdf)

Assuring Pluripotency in ATCC iPSCs (644 KB/.pdf)

Bone Marrow-Derived Mesenchymal Stem Cells (316 KB/.pdf)

Brain Tumor Cell Lines (657 KB/.pdf)

Breast Cancer Resource Book (988 KB/.pdf)

Breast and Gynecological Tumor Cell Lines (335 KB/.pdf)

Cancer Immunology Research Tools (304 KB/.pdf)

Cancer Resources (370 KB/.pdf)

Cell Line Authentication Test Recommendations (483 KB/.pdf)

Cell Lines by Gene Mutation Brochure (1.68 MB/.pdf)

Certified Reference Material (300 KB/.pdf)

Complete Cell Culturing Solutions for iPSCs (348KB MB/.pdf)

CoolCell® LX Alcohol-Free Cryopreservation Containers (463KB/.pdf)

Epithelial-mesenchymal Transition Reporter Cell Line (248KB/.pdf)

Essential Neural Cell Lines (69KB/.pdf)

Exosomes (141KB/.pdf)

Gastrointestinal Tumor Cell Lines (344 KB/.pdf)

Genetic Alteration Panels Brochure (1.8 MB/.pdf)

Genitourinary Tumor Cell Lines (783 KB/.pdf)

Genomic DNA from Human Cell Lines (256 KB/.pdf)

hTERT Immortalized Primary Cells (1.34MB/.pdf)

hTERT Immortalized Renal Proximal Tubule Epithelial Cells (388 KB/.pdf)

Hybridomas by Antigenic Determinant (804 KB/.pdf)

Isogenic Cell Lines (300KB/.pdf)

Luciferase Cell Lines (1.48 MB/.pdf)

Lung Tumor Cell Lines (808 KB/.pdf)

Maintaining High Standards in Cell Culture (1 MB/.pdf)

Neural Progenitor Cells (308KB/.pdf)

Neurobiology Research Tools (308KB/.pdf)

p53 Hotspot Mutation Cell Panels Brochure (1.85 MB/.pdf)

Primary Cardiovascular Cells (272 KB/.pdf)

Primary Cell Solutions Brochure (1.42 MB/.pdf)

Primary Cell Solutions Collection (686 KB/.pdf)

Primary Human Dermatological Cells (288 KB/.pdf)

Primary Human Immune Cells (275 KB/.pdf)

Primary Human Respiratory Cells (275 KB/.pdf)

Purified And Quantitative Genomic Cell Line DNA (996KB/.pdf)

Reliablue™ Cell Viability Reagent: A one-step reagent for assessment of cell viability, proliferation, and metabolism (516KB/.pdf)

Stem Cell Solutions Brochure (279 KB/.pdf)

Toxicology Portfolio (2.37 MB/.pdf)

Toxicology Tools (340 KB/.pdf)

Transfection reagents for nucleic acid transfer into ATCC cells (300 KB/.pdf)

Tumor/Normal Matched Cell Line Pairs (362 KB/.pdf)

Tumor Cell Panels Brochure (2 MB/.pdf)

Application Notes

Application Note No. 1: 3T3-L3 Cells — 3T3-L1 cells lose contact inhibition and adipogenic potential when maintained in cult ure at confluence. (972KB/.pdf)

Application Note No. 2: Tips for successful sub-culturing: Dissociation Method — Tailoring your dissociation method to your cell line. (523KB/.pdf)

Application Note No. 4: CellMatrix Basement Membrane Gel — CellMatrix Basement Membrane Gel supports in vitro angiogenesis assays. (980KB/.pdf)

Application Note No. 6: Universal Mycoplasma Detection Kit — check your cultures for mycoplasma contamination. (972KB/.pdf)

Application Note No. 8: Telomerase-immortalized microvascular endothelial (TIME) Cells — Growth rate and endothelial character are better maintained in ATCC® VCBM -VEGF medium. (968KB/.pdf)

Application Note No. 14: Human Bronchial/Tracheal Epithelial Cells  — Improving Functional Studies. (530KB/.pdf)

Application Note No. 16: Differentiation and expansion of hematopoietic precursor cells from bone-marrow derived CD34+ progenitors — (512KB/.pdf)

Application Note No. 17: htert-immortalized and Primary Keratinocytes Differentiate into Epidermal Structures in 3d Organotypic Culture (500KB/.pdf)

Application Note No. 19:  In vitro angiogenesis assay using the ATCC® Angio-Ready™ System (4.11MB/.pdf)

Application Note No. 21:  In vitro differentiation of macrophages and dendritic cells from primary human CD14+ monocytes (510KB/.pdf)

Application Note No. 23: Comprehensive Gene Expression Analysis and Neurotoxicity Testing of Human iPSC-Derived Neural Progenitor Cells and Neurons (2.8MB/.pdf)

Application Note No. 24: Establishment and Characterization of a Kidney-Drug Interaction Model by Stably Expressing hOAT1 in HEK 293T/17 Cells (732KB/.pdf)

Application Note No. 25: The Generation of an EML4-ALK Fusion NSCLC Isogenic Cell Line Relevant for Drug Discovery and Development (432KB/.pdf)

Application Note No. 26: Directed differentiation of gastrointestinal epithelial organoids using ATCC® CellMatrix Basement Membrane from multiple human ATCC iPSC lines (748 KB/.pdf)

Application Note No. 31: A simple and rapid alternative to 51chromium or fluorescent dye loading for quantification of natural killer cell activity: ATCC® K-562-GFP™ cells (322KB/.pdf)

Application Note No. 32: Genetically Modified Renal Proximal Tubule Epithelial Cells (3.63MB/.pdf)

Application Note No. 33: Protocol for the thawing, expansion, and cryopreservation of mouse small intestinal organoids (2.37MB/.pdf)

Video Resources

ATCC Excellence in Research Recorded Webinars — Each webinar features highly experienced Scientists who demonstrates how to utilize ATCC products and services to advance your research!

Published Articles

The Scientist — Sharing Made Easy

GEN —  Safeguarding Biological Material

Drug Discovery News — Working together to eradicate cell line misidentification and contamination: New practices and policy

Technical Bulletins and Culture Tips

How to Revive Cultures

Technical Bulletin No. 3: Cryogenic Preservation of Animal Cells (50.2KB/.pdf)

Technical Bulletin No. 4: Guide to Subculturing Cell Line Monolayers (47.8KB/.pdf)

Technical Bulletin No. 7: Passage Number Effects In Cell Lines (220KB/.pdf)

Technical Bulletin No. 8: Cell Line Authentication Test Recommendations (483KB/.pdf)

Technical Bulletin No. 9: Chemically-induced Differentiation of ATCC® CL-173™ (3T3-L1) Using Single Component Commercially Available Reagents  (61KB/.pdf)

Technical Bulletin No. 10: Lymphocyte Transformation Using ATCC® VR-1492™ (241KB/.pdf)

Cell Authentication

Cell Authentication — STR Essentials

Cell Lines Misidentification: the beginning of the end

Cryogenic Preservation of Animal Cells (50.2KB/.pdf)

Guide to Subculturing Cell Line Monolayers (47.8KB/.pdf)

Guidelines for Testing and Publishing (47.8KB/.pdf)

Misidentified Cell lines

Mycoplasma Detection (585 KB/.pdf)

Passage Number Effects In Cell lines (377KB/.pdf)

STR Profiling (471 KB/.pdf)

The Costs of Using Unauthenticated, Over-Passaged Cells (391 KB/.pdf)

Titered Mycoplasma Strains Panel (906KB/.pdf)

Cell Culture Media and Kits

Comparison of the MTT and XTT Cell Proliferation Assay Kits (561KB/.pdf)

Primary Cell Culture Media, Growth Kits and Reagents

Stem Cell Culture Reagents and Media

References and Additional Publications

Human Primary Cell Infographic (2.62MB/.pdf)

Selected References Regarding Cell Culturing (662KB/.pdf)

Newsletters

ATCC technical documents can be downloaded using Adobe Acrobat Reader

While ATCC uses reasonable efforts to include accurate and up-to-date information in this technical help section, we make no warranties or representations as to its accuracy. ATCC is not responsible for any errors or omissions or for the results obtained from the use of this information. Content of the site is subject to change without notice.