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Technical Documents

Gloved hand holding mini pipette over petri dish containing bacteria colonies

Specific instructions on techniques for using ATCC products

Culturing, maintaining, and cryopreserving microbial cultures and cell lines can be challenging at times. Explore our collection of technical documents to access detailed information on the techniques used by ATCC experts for the authentication, application, and handling of our products.

Gold, bean-shaped pods of Mycoplasma pneumoniae. Technical document

Cell Line Authentication Test Recommendations

Authentication of a cell line is the process by which a line’s identity is verified and shown to be free of contamination from other cell lines and microbes.

Red and blue fibroblasts. Technical document

A Chemically-induced Method of Adipogenesis

3T3-L1 materials from at least two different distribution lots were tested for their ability to differentiate in response to a mixture of insulin, dexamethasone, and IBMX (methylisobutylxanthine). The assay was established according to published protocols and is relatively easy to perform.

Gloved hand covered in ice, holding frozen vial, above cryopreservation tank filled with boxes of frozen vials. Technical document

Cryogenic Storage of Animal Cells

Cryogenic preservation of cell cultures is widely used to maintain reserves of cell cultures. Besides providing a valuable back-up supply, properly stored cultures also reduce alterations in or loss of culture characteristics.

Blue and white parts of RNA strand. Technical document

The ATCC Genome Portal: Our Approach to Whole-genome Sequencing

Discover the features of the ATCC Genome Portal and understand the DNA extraction, sequencing, and bioinformatic methods we use to produce high-quality, reference-grade genomes.

Small, fluorescent blue and white prostate cells. Technical document

Guide to Subculturing Cell Line Monolayers

Most cell lines and primary cell cultures grow as a single thickness cell layer (monolayer) or sheet attached to glass or specially treated plastic substrates. In order to keep cultures healthy and actively growing it is usually necessary to subculture them at regular intervals.

T-lymphocytes cancer cell. Technical document

Lymphocyte Transformation Using ATCC VR-1492

We describe the procedure for transformation with Epstein-Barr virus (EBV) that has a 95% success rate.

Blue and red prostate adenocarcinoma. Technical document

Passage Number Effects in Cell Lines

The degree of subculturing a cell line has undergone is often expressed as “passage number,” which can generally be thought of as the number of times cells have been transferred from vessel-to-vessel.

Pink, flower-like strands of Aspergillus fumigatus fungus. Technical document

Preservation and Recovery of Filamentous Fungi

Preservation methods for filamentous fungi vary depending on the type and degree of sporulation. Read this technical document to learn more.

Petri dishes containing purple and green media. Technical document

Reference Strains: How Many Passages are Too Many?

This technical document will attempt to clear up some of the confusion about passage and microbial culture maintenance and provide some definitions and recommendations.


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Application Notes

Read our application notes for high-quality data exploring the development, validation, and application of ATCC products.

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Culture Guides

Download these useful guides and start with fresh authenticated cells and strains from ATCC to achieve the best results.

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