Technical Documents

Authentication of a cell line is the process by which a line’s identity is verified and shown to be free of contamination from other cell lines and microbes.

3T3-L1 materials from at least two different distribution lots were tested for their ability to differentiate in response to a mixture of insulin, dexamethasone, and IBMX (methylisobutylxanthine). The assay was established according to published protocols and is relatively easy to perform.

Cryogenic preservation of cell cultures is widely used to maintain reserves of cell cultures. Besides providing a valuable back-up supply, properly stored cultures also reduce alterations in or loss of culture characteristics.

Discover the features of the ATCC Genome Portal and understand the DNA extraction, sequencing, and bioinformatic methods we use to produce high-quality, reference-grade genomes.

Most cell lines and primary cell cultures grow as a single thickness cell layer (monolayer) or sheet attached to glass or specially treated plastic substrates. In order to keep cultures healthy and actively growing it is usually necessary to subculture them at regular intervals.

We describe the procedure for transformation with Epstein-Barr virus (EBV) that has a 95% success rate.

The degree of subculturing a cell line has undergone is often expressed as “passage number,” which can generally be thought of as the number of times cells have been transferred from vessel-to-vessel.

Preservation methods for filamentous fungi vary depending on the type and degree of sporulation. Read this technical document to learn more.

This technical document will attempt to clear up some of the confusion about passage and microbial culture maintenance and provide some definitions and recommendations.