Technical Documents

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Culturing, maintaining, and cryopreserving microbial cultures and cell lines can be challenging at times. Explore our collection of technical documents to access detailed information on the techniques used by ATCC experts for the authentication, application, and handling of our products.

Red cytokeratin-positive cells. Technical document

Cell Line Authentication Test Recommendations

Authentication of a cell line is the sum of the process by which a line’s identity is verified and shown to be free of contamination from other cell lines and microbes.

Green and purple Cyto-01 retinal epithelial cells. Technical document

Chemically Induced Differentiation of ATCC CL-173 (3T3-L1) Using Single-Component Commercially Available Reagents

3T3-L1 materials from at least two different distribution lots were tested for their ability to differentiate in response to a mixture of insulin, dexamethasone, and IBMX (methylisobutylxanthine). The assay was established according to published protocols and is relatively easy to perform.

Spherical, fluorescent teal-blue hepatocyte epithelial cells. Technical document

Cryogenic Storage of Animal Cells

Cryogenic preservation (storage below -130°C) of cell cultures is widely used to maintain reserves of cell cultures. Besides providing a valuable back-up supply, properly stored cultures also reduce alterations in or loss of culture characteristics.

Blue and white parts of RNA strand. Technical document

The ATCC Genome Portal: Our Approach to Whole-genome Sequencing

Discover the features of the ATCC Genome Portal and understand the DNA extraction, sequencing, and bioinformatic methods we use to produce high-quality, reference-grade genomes.

Small, fluorescent blue and white prostate cells. Technical document

Guide to Subculturing Cell Line Monolayers

Most cell lines and primary cell cultures grow as a single thickness cell layer (monolayer) or sheet attached to glass or specially treated plastic substrates. In order to keep cultures healthy and actively growing it is usually necessary to subculture them at regular intervals.

T-lymphocytes cancer cell. Technical document

Lymphocyte Transformation Using ATCC VR-1492

This technical document describes the procedure recommended for transformation with Epstein-Barr virus (EBV) as described by Caputo et al. in 1995, which is reported to have a success rate of 95%.

Red and yellow conditionally reprogrammed organoid cells. Technical document

Passage Number Effects in Cell Lines

The degree of subculturing a cell line has undergone is often expressed as “passage number,” which can generally be thought of as the number of times cells have been transferred from vessel-to-vessel.

Pink, flower-like strands of Aspergillus fumigatus fungus. Technical document

Preservation and Recovery of Filamentous Fungi

Preservation methods for filamentous fungi vary depending on the type and degree of sporulation.

Several petri dishes containing media of various colors. Technical document

Reference Strains: How many passages are too many?

This technical document will attempt to clear up some of the confusion about passage and microbial culture maintenance and provide some definitions and recommendations.


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