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Bovine viral diarrhea virus 1

VR-534

Product category
Viruses
Product type
Animal virus
Classification
Flaviviridae, Pestivirus
Strain designation
NADL
Common name
Bovine viral diarrhea virus 1
Type strain
No
Isolation source
Spleen of a naturally occurring fatal case of BVD in the closed herd at National Animal Disease Laboratory.
Geographical isolation
United States; Iowa
Applications
Agricultural research
Product format
Frozen
Storage conditions
-70°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

BT

CRL-1390

Price: $495.00 ea

Dulbecco's Modified Eagle's Medium (DMEM)

30-2002

Price: $22.00 ea

Dimethylsulfoxide (DMSO)

4-X

Price: $49.00 ea

Detailed product information

General

Specific applications
REFERENCE STRAIN: Western Hemisphere Committee on Non-primate Animal Virus Characterization (WHO).

Characteristics

Comments
REFERENCE STRAIN: Western Hemisphere Committee on Non-primate Animal Virus Characterization (WHO). VR-1422 is a high titer variant of this strain.
Mycoplasma contamination
Not detected

Handling information

Host
primary bovine embryonic kidney cells
Effects
cell rounding; cell sloughing; CPE
Complete medium
DMEM (ATCC® 30-2002™) + 10% Horse Serum (ATCC® 30-2040™)
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
2-6 days
Recommendation for infection
For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Handling notes
BVD viruses cross-fluoresce and have a

       common soluble antigen with hog cholera viruses.

       The buoyant density of BVD viruses is 1.15 g/mL     

       and the size of the virion is thought to be 80-100

       millimicrons. This preparation may be readily

       adapted to growth in PK-15 cells (ATCC CCL-33)

       (Malmquist, W.A. et al., American Journal of  

       Veterinary Research 26: 1316, 1965). ATCC VR-

       1422 is a variant of this strain that is amenable to

       being grown at higher titers.  VR-534 is a

       recognized reference strain by the Western

       Hemisphere Committee on Non-primate Animal

       Virus Characterization (WHO).

Key abbreviations
BEK, Bovine embryonic kidney cells
BT, Bovine turbinate cells
CO2, Carbon dioxide
CPE, Cytopathic effect
DMEM, Dulbecco's Modified Eagle's Medium
HS, Horse serum
PK-15, Porcine kidney cells, clone 15
Pr, Primary
TCID[50], Median tissue culture infective dose
USDA, United States Department of Agriculture
WHO, World Health Organization

History

Deposited as
Bovine viral diarrhea
Depositors
AL Fernelius
Type of isolate
Animal
Year of origin
1962
Special collection
NCRR Contract

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors

For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. We cannot ship this item until we receive this permit.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Vassilev VB, et al. Authentic and chimeric full-length genomic cDNA clones of bovine viral diarrhea virus that yield infectious transcripts. J. Virol. 71: 471-478, 1997. PubMed: 8985373

Gutekunst KE. . Can. J. Comp. Med. Vet. Sci. 27: 121, 1963.

Gutekunst KE. . Can. J. Comp. Med. Vet. Sci. 28: 19, 1963.

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