Primary CD56+ NK Cells
PCS-800-019 ™
Primary CD56+ NK Cells exhibit lymphoid morphology and were isolated from the peripheral blood of a donor. This cell has applications in compound screening and immune function assays.
- Product category
-
Human cells
- Product type
-
Primary cell
- Organism
-
Homo sapiens, human
- Morphology
- lymphoid
- Tissue
- Blood
- Disease
-
Normal
- Applications
-
3D cell cultureImmunologyToxicologyImmuno-oncologyCell and gene therapy (CGT) development
- Product format
- Frozen
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
All tissues used for isolation are obtained under informed consent and conform to HIPAA regulations to protect the privacy of the donor’s Personally Identifiable Information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry Human immunodeficiency virus (HIV) and other bloodborne pathogens. With infectious virus assays or viral antigen assays, even a negative test result may not exclude the possibility of the existence of a latent viral genome or infectious viral particles below the lower limit of detection of that assay.
ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Handle as a potentially biohazardous material using universal precautions. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Detailed product information
General
- Specific applications
-
Research purposes, compound screening, immune function assays such as the Natural Killer Cell Assay.
See Application Note No. 31: A simple and rapid alternative to 51chromium or fluorescent dye loading for quantification of natural killer cell activity: ATCC K-562-GFP cells for more information.
Characteristics
- Cells per vial
- ≥ 1.0 x 106
- Volume
- 1.0 mL
- Growth properties
- Suspension
- Age
- lot-specific
- Ethnicity
- Lot-specific
- Gender
- Lot-specific
- Comments
-
CD56+ Natural killer (NK) cells are the major effectors of the innate immune
system and are important in host defense, cancer, and autoimmunity.
CD56+ Natural Killer Cells are isolated from mononuclear cells by negative selection using immunomagnetic cell separation procedures.
ATCC CD56+ Natural Killer cells are tested by flow cytometry for the following surface markers:- CD45+: ≥ 90%
- CD56+: ≥ 90%
- CD56+CD3-: Report results
- CD56+CD16+: Report results
Handling information
- Unpacking and storage instructions
-
- Check all containers for leakage or breakage.
- Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
- Complete medium
- Natural Killer Cells have a limited lifespan in culture and should be thawed immediately and only prior to their intended use in application specific media. ATCC recommends thawing them in RPMI-1640 (ATCC 30-2001). ATCC does not recommend maintaining Natural Killer Cells in culture in the absence of application-specific growth factors.
- Temperature
-
37°C
- Atmosphere
-
95% Air, 5% CO2
- Handling procedure
-
Refer to the batch specific information for the total number of viable cells recovered from this lot of ATCC PCS-800-016.
- Rapidly thaw the vial in a 37oC water bath.
- Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by spraying with 70% ethanol. All operations from this point onward should be carried out under strict aseptic conditions.
- Carefully transfer the contents of the vial to a conical tube containing 5mL HBSS (without Ca2+ or Mg2+) containing 10% Human AB serum or 10% Human Serum Albumin.
- Rinse the vial with additional HBSS and transfer to the conical tube.
- Bring the volume in the cryovial up to 10 mL with HBSS containing 10% HSA.
- Take an aliquot for counting.
- Centrifuge the cell suspension at 300 x g for 5 minutes at room temperature.
- Carefully aspirate the supernatant without disturbing the pellet.
- Gently re-suspend the pellet in an appropriate buffer/medium such as RPMI-1640 containing 10% HSA.
Quality control specifications
- Virus testing
-
Human Immunodeficiency virus (HIV): Not detectedHepatitis C virus (HCV): Not detectedHepatitis B virus (HBV): Not detected
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Images
Frequently Asked Questions
References
Curated Citations
Chang H, et al. GAS-Luc2 Reporter Cell Lines for Immune Checkpoint Drug Screening in Solid Tumors. Cancers 2024, 16, 1965. https://doi.org/10.3390/cancers16111965
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