Primary Pulmonary Artery Smooth Muscle Cells; Normal, Human (PASMC)
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All tissues used for isolation are obtained under informed consent and conform to HIPAA regulations to protect the privacy of the donor’s Personally Identifiable Information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry Human immunodeficiency virus (HIV) and other bloodborne pathogens. With infectious virus assays or viral antigen assays, even a negative test result may not exclude the possibility of the existence of a latent viral genome or infectious viral particles below the lower limit of detection of that assay.
ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Handle as a potentially biohazardous material using universal precautions. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
The PASMC cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency.A complete solution to propagate Vascular Smooth Muscle Cells in low serum conditions.
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Kim HJ, et al. Wall Stretch and Thromboxane A2 Activate No Synthase (eNOS) in Pulmonary Arterial Smooth Muscle Cells Via H2O2 and Akt-Dependent Phosphorylation. Pflugers Arch 4(468):705-16, 2016. PubMed: 26729266
Cheng GS, et al. Bone Marrow-Derived Mesenchymal Stem Cells Modified with IGFBP-3 Inhibit the Proliferation of Pulmonary Artery Smooth Muscle Cells. Int J Mol Med, 1(39):223-30, 2017. PubMed: 27959432
Chakraborty R, et al. Characterization of GPCR Signaling in Hypoxia. Methods Cell Biol 142:101-10, 2017. PubMed: 28964329
Hao M, et al. Galectin-3 inhibition ameliorates hypoxia-induced pulmonary artery hypertension. Mol Med Rep 15(1):160-168, 2017. PubMed: 27959409
Wei C, et al. Exogenous Spermine Inhibits the Proliferation of Human Pulmonary Artery Smooth Muscle Cells Caused by Chemically-Induced Hypoxia Via the Suppression of the ERK1/2- and PI3K/AKT-Associated Pathways. Int J Mol Med 1(37):39-46, 2016. PubMed: 26572277