Vascular Smooth Muscle Cell Growth Kit
PCS-100-042 ™
PCS-100-042 ™
The Vascular Smooth Muscle Cell Growth Kit contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete cell culture environment for smooth muscle cells isolated from large vessels. The final concentration for each component in complete vascular smooth muscle cell growth medium is as follows:
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Unpacking and Storage Instructions
Preparation of Complete Growth Media
Table 1. Vascular Smooth Muscle Cell Growth Kit Components
Component | Volume | Final Concentration |
rh FGF-basic | 0.5 mL | 5 ng/mL |
rh Insulin | 0.5 mL | 5 µg/mL |
Ascorbic acid | 0.5 mL | 50 µg/mL |
L-glutamine | 25.0 mL | 10 mM |
rh EGF | 0.5 mL | 5 ng/mL |
Fetal Bovine Serum | 25.0 mL | 5% |
Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 2.
Table 2. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)
Component | Volume | Final Concentration |
Gentamicin-Amphotericin B Solution | 0.5 mL | Gentamicin: 10 µg/mL Amphotericin B: 0.25 µg/mL |
Penicillin-Streptomycin-Amphotericin B Solution | 0.5 mL | Penicillin: 10 Units/mL Streptomycin: 10 µg/mL Amphotericin B: 25 ng/mL |
Phenol Red | 0.5 mL | 33 µM |
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