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Product category
Animal cells
Product type
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell type
hybridoma: b lymphocyte
Product format
Mission Collection Item
This is a Mission Collection Item.


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-22.
Tested and found negative for ectromelia virus (mousepox).


Growth properties
Spleen cells were fused with P3X63Ag8 myeloma cells.
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-22.
Yes, in pristane primed BALB/c mice
Genes expressed
immunoglobulin, monoclonal antibody, against human T cell (human T lymphocyte); also reacts with human transferrin receptor
IgG1, kappa light chain
Animals were immunized with HSB-2 cells (ATCC CCL-120.1, an acute lymphocytic T leukemia cell line).
Spleen cells were fused with P3X63Ag8 myeloma cells.
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-22.
The antibody recognizes a 90000 dalton cell surface antigen (the same as that recognized by OKT 9, ATCC CRL-8021).
The antibody precipitates human transferrin receptor.
It does not fix complement.
Tested and found negative for ectromelia virus (mousepox).

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.
  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
Dulbecco's modified Eagle's medium, 90%; fetal bovine serum, 10%
Handling procedure
Vol./Ampule: 1.0 mL.
Recommended Handling Upon Receipt: Initiate culture as soon as possible upon receipt. Thaw by rapid agitation in 37°C water bath. See instructions on back.
Dilute ampule contents with culture medium (see batch data above). Add fresh
medium (depending on cell density) every 2-3 days.
Subculturing procedure
Medium Renewal: Every 2 to 3 days
This line has carried by passage as ascites in pristane primed (0.5 ml, 7 days prior to injection of cells) mice.
The mice are injected with 0.3 to 0.5 ml of cells, and the ascites is collected 7 to 10 days later.
Recent information from the donor indicates that the cells are readily grown in culture using the above medium.
Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 5 X 10 exp4 cells/ml and do not allow the cells to exceed 8 X 10 exp5 cells/ml.

Quality control specifications

Mycoplasma contamination
Not detected


Deposited as
mouse (B cell); mouse (myeloma)
A Fauci, BF Haynes
Special collection
Hybridoma Bank (HB)

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Haynes BF, et al. Characterization of a monoclonal antibody (5E9) that defines a human cell surface antigen of cell activation. J. Immunol. 127: 347-351, 1981. PubMed: 6787129

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