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Aspergillus brasiliensis Varga et al.

CRM-16404

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Lyophilized fungal spores and mycelium residue in a vial
Product category
Fungi
Product type
Certified reference material
Classification
Fungi, Dikarya, Ascomycota, Pezizomycotina, Eurotiomycetes, Eurotiomycetidae, Eurotiales, Aspergillaceae, Aspergillus
Strain designation
WLRI 034(120) [CBS 733.88, DSM 1387, DSM 1988, IFO 9455, IMI 149007, NCPF 2275]
Type strain
No
Genome sequenced strain
Yes
Isolation source
Blueberry
Geographical isolation
United States; North Carolina
Applications
Assay development
Media testing
Quality control
Product format
Freeze-dried
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Documentation

Biosafety Icon Biosafety Level 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. 
assay of antimicrobial preservatives
media testing
preparatory test control
quality control strain
sterility testing
testing
testing fungicides
transformation host
validation control
Antimicrobial effectiveness testing RefU.S. Pharmacopeia General Chapters:<51> ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011
Microbial enumeration testing RefU.S. Pharmacopeia General Chapters: <61> Microbial Examination of Nonsterile Products: Microbial Enumeration Tests. Rockville, MD USP34-NF29, 2011
This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified.
Preceptrol
No

Characteristics

Morphology
Colonies initially white or yellowish, mycelium growing rapidly (to cover plate in 5 to 10 days), soon producing dense layer of erect smooth-stiped, thick-walled conidiophores terminated by globose vesicles bearing phialides (uniseriate) or (commonly) metulae with phialides (biseriate) which produce dry chains of conidia. Revers of plate pale to grayish or greenish yellow, often showing radiating ridges in mycelium. Spore heads radiate, sometimes dividing into columns with age, initially pale, becoming dark brown to black. Individual conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) micrometers in diameter.
Comments
Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO 17034 accredited process.

This strain was recently re-named as Aspergillus brasiliensis according to the reference Varga et al. 2007. 

Use of impedance for preservative efficacy testing

Handling information

Medium
Temperature
20-25°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
  1. Open vial according to enclosed instructions.
  2. From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet to rehydrate it.  Stir to form a suspension.
  3. Aseptically transfer the rehydrated pellet (suspension) back into the test tube with the sterile distilled water.  Mix well.
  4. Let the test tube sit at room temperature (~23 °C) for at least 2 hours, if not overnight.
  5. Mix the suspension well.  Use several drops to inoculate a test tube or slant or a plate with recommended medium.
  6. Incubate the test tube or plate at the temperature recommended for 2-11 days.

 

Handling notes
Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

 

Sporulation may be inhibited in plates sealed completely with tape.  Colonies grown directly from rehydrated spores may exhibit sectoring, with areas of varying levels of sporulation.  Intermittent light exposure helps sporulation after incubating 48 hours at 30°C.

 

This strain was identified as belonging to the new species Aspergillus brasiliensis, described in the following two publications:

  1. Varga et al. 2007. Int. J. Syst. Evol. Microbiol. 57:1925-1932.
  2. Houseknecht, J., Stamenova, E., Suh, S.-O., Beck, B., McKee, M. & Zhou. J. 2008. Reclassification of ATCC® 16404™ and ATCC® 9642™ as Aspergillus brasiliensis. Pharmaceutical Microbiology Forum Newsletter 14: 2-8.

Quality control specifications

Verification method
Whole-genome Sequencing
Quality accreditation

Manufactured under an ISO 17034 accredited process

    ACLASS Accredited Reference Material Producer AR-1384

History

Deposited as
Aspergillus niger van Tieghem, anamorph
Depositors
SM Ringel
Type of isolate
Food & Beverage; Plant
Special collection
Certified Reference Materials
Cross references
GenBank KU729174 D1/D2 region of 28S rRNA gene
GenBank FJ195348 ITS including 5.8S rRNA gene

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Certified Reference Material produced under an ISO 17034 accredited process.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Connolly P, et al. The use of impedance for preservative efficacy testing of pharmaceuticals and cosmetic products. J. Appl. Bacteriol. 76: 68-74, 1994. PubMed: 8144407

Wnendt S, et al. Molecular cloning, sequence analysis and expression of the gene encoding an antifungal-protein from Aspergillus giganteus. Curr. Genet. 25: 519-523, 1994. PubMed: 8082203

ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA

British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 2003

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

View All Curated Citations for this Product

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