U-CH17M (ATCC® CRL-3402)

Organism: Homo sapiens, human  /  Tissue: soft tissue metastasis  /  Disease: chordoma; malignant

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Organism Homo sapiens, human
Tissue soft tissue metastasis
Product Format frozen 1.0 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease chordoma; malignant
Age 38 years old
Gender male
Ethnicity Caucasian
Applications
A cell culture system model for observing tumor diversity and progression.
Use as a model for chordoma and for predicting the clinical performance of cancer drugs.

The Chordoma Foundation may be able to offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information.
Images
Comments Tumor type/stage/grade: pT2b, V0, L0, Pn, N0(0/3), M1
Complete Growth Medium

The base medium for this cell line is Iscove's Modified Dulbecco's Medium (IMDM; ATCC 30-2005): RPMI-1640 Medium (ATCC 30-2001) at a 4 to 1 ratio. To 500 mL IMDM:RPMI 1640 (4:1) add the following components to make the complete medium:

  • 56 mL  Fetal Bovine Serum (FBS; ATCC 30-2020), final concentration of 10%
  • 5.6 mL L-glutamine from stock 200 mM (ATCC 30-2214)
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    Cultures can be established between 2 x 104 and 4 x 104 viable cells/cm2.
  6. Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 1 X 104 and 1.5 X 105 cell/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Culture media + 5% DMSO (ATCC 4-X)
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial Approximately 1.5 to 2.5 x 106
Volume 1.0 mL
STR Profile
Amelogenin: X,Y
CSF1PO: 12,14
D13S317: 11
D16S539: 13
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 11
vWA: 14,17
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viability ≥ 50%
Population Doubling Time Per depositor: 8 - 10 days
Name of Depositor University of Ulm, Germany
Year of Origin 2017
References

Jager D, et al. U-CH17P, -M and –S, a new cell culture system for tumor diversity and progression in chordoma. Int J Cancer 142(7): 1369-78, 2017. PubMed: 29148152

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Jager D, et al. U-CH17P, -M and –S, a new cell culture system for tumor diversity and progression in chordoma. Int J Cancer 142(7): 1369-78, 2017. PubMed: 29148152