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Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 43300)

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Strain Designations: F-182  /  Type Strain: no  /  Biosafety Level: 2

Verified By Whole-genome Sequencing
Strain Designations F-182
Evaluation of Mueller-Hinton agar
Susceptibility disc testing
Susceptibility testing
Drug discovery
Clinical isolate, Kansas
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Genotype pvl negative
Type Strain no
Antibiotic Resistance Methicillin resistant
Oxacillin resistant
Comments MRSA
SCCmec: Type II
spa type Ridom: t007
spa type Kreiswirth: WGKKKKAOM
pvl gene amplification: Negative

Phenotypic and genotypic information for ATCC MRSA strains, such as SCCmec, PFGE, or spa typing, is provided for informational purposes only. This information was compiled from testing performed by one or more sources, including ATCC, strain depositor, published data, or contracted research.
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype pvl negative
Name of Depositor C Thornsberry
Clinical isolate, Kansas

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3.

Baker CN, Tenover FC. Evaluation of Alamar colorimetric broth microdilution susceptibility testing method for staphylocci and enterococci. J. Clin. Microbiol. 34: 2654-2659, 1996. PubMed: 8897159

Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P.

Medical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002

MicroScan Dried Gram Positive Panel QC Set. Dade/Siemens.

BD GeneOhm MRSA ACP. BD Becton Dickinson.

BD GeneOhm StaphSR. BD Becton Dickinson.

BD MAX™ MRSA Assay. BD Becton Dickinson.

BD MAX™ StaphSR Assay. BD Becton Dickinson.

Monecke S, et al. A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS One 6(4): e17936, 2011. PubMed: 21494333

Cross References

Nucleotide (GenBank) : AF298796 Staphylococcus aureus translation elongation factor Tu (tuf) gene, partial cds.