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Kocuria rhizophila Kovacs et al.


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Kocuria rhizophila strain FDA strain PCI 1001 was isolated from soil. This whole-genome sequenced bacterial strain is recommended as a test microorganism in USP chapters <71> and <81> and it can be used as a quality control strain in sterility testing and susceptibility disc testing.
Product category
Strain designation
FDA strain PCI 1001
Type strain
Genome sequenced strain
Former name
Micrococcus luteus (Schroeter) Cohn
Isolation source
Food testing
Media testing
Quality control
Pharmaceutical testing
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Assay of amoxicillin
Assay of ampicillin
Assay of clindamycin
Assay of cyclacillin
Assay of erythromycin
Assay of fungicide residues
Assay of lincomycin
Assay of novobiocin
Assay of oleandomycin
Assay of penicillamine
Food testing
Pharmaceutical and Personal Care
Assay of penicillin
Assay of rifamycins rifamycin
Assay of tylosin
Media testing
Membrane filter testing
Produces 6-aminopenicillanic acid
Produces sorbosone L-sorbosone
Quality control strain
Sterility testing
Susceptibility disc testing chloramphenicol
Susceptibility disc testing doxycycline hydramycin
Susceptibility disc testing tetracycline
Recommended as a test microorganism for EP 2.7.2 Microbiological Assays of Antibiotics
Recommended as a test microorganism for EP Monographs
Recommended as a test microorganism for JP 16 Monographs
Recommended as a test microorganism for USP <71> Sterility Tests
Recommended as a test microorganism for USP <81> Antibiotics-Microbial Assays


Genome sequenced strain
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Handling procedure
  1. Open vial according to enclosed instructions or visit for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #18 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #18 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #18 plate and/or #18 agar slant.
  4. Incubate at 30°C for 24-48 hours.
Handling notes

The strain is yellow-pigmented. If grown on Nutrient Agar (BD 213000) or Tryptic Soy Agar (BD 236950) with 5% defibrinated sheep blood, two colony types may be observed. These appear to be rough and smooth variants. This variation is less apparent on #18 plates.
The rough colonies are reported to be much more difficult to suspend in liquid for testing and have subtly different antibiotic susceptibilities than the smooth phenotype. Therefore, it is advisable to select smooth colonies when transferring this culture.

This strain is available as a certified reference material, ATCC® CRM-9341™.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Sarcina lutea (Schroeter) Schroeter
Chain of custody
ATCC <-- FDA <-- ER Squibb & Sons
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Type of isolate
Special collection
NSF - Bacteriology
Cross references
GenBank AP009152 Kocuria rhizophila DC2201 DNA, complete genome.
GenBank AB103093 Micrococcus luteus genes for 16S rRNA, 16S-23S ISR, 23S rRNA, partial and complete sequence, strain:ATCC 9341.
GenBank AF542072 Kocuria rhizophila 16S ribosomal RNA gene, partial sequence.
GenBank K02683 M. luteus (ATCC 9341) 5S ribosomal RNA.
GenBank U10878 Micrococcus luteus FtsZ (ftsZ) gene, partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Makover S, Pruess DL. Method for producing L-sorbosone. US Patent 3,912,592 dated Oct 14 1975

British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003

European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997

U.S. Food & Drug Administration Inhibitory substances in milk, Sarcina lutea cylinder plate methodIn: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed.Gaithersburg, MD. AOAC International pp. 20.01-20.04, 1995

US Food & Drug Administration. GENERAL BIOLOGICAL PRODUCTS STANDARDS; General Provisions; Sterility Code of Federal Regulations Title 21: 21CFR610.12, Subpart B, 2005

View All Curated Citations for this Product

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