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Clostridium perfringens (Veillon and Zuber) Hauduroy et al.


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Clostridium perfringens strain NCTC 8237 [ATCC 19408, CIP 103 409, CN 1491, NCIB 6125, NCTC 6125, S 107] is a whole-genome sequenced bacterial type strain. This toxigenic culture has applications in agricultural research, bioinformatics, quality control, media testing, and water testing.
Product category
Product type
Preceptrol culture
Strain designation
NCTC 8237 [ATCC 19408, CIP 103 409, CN 1491, NCIB 6125, NCTC 6125, S 107]
Type strain
Genome sequenced strain
Agricultural research
Media testing
Quality control
Water testing
Product format
Storage conditions
2°C to 8°C
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Biosafety Icon Biosafety Level 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Media testing
Produces hemolysins
Produces lethal toxins
Produces phospholipase C lecithinase C
Quality control strain
Reference material
Control strain for anaerobe identification
Quality control strain for API, BBL, and MicroScan [Reg TM] products
Water testing


Toxin genes
cpa (Alpha toxin) positive
etx (Epsilon toxin) negative
pfoA (Perfringolysin O) positive
Penicillin-binding protein profile
Type A, alpha-toxigenic
The presence of cpa and pfoA genes was confirmed by PCR.
Genome sequenced strain
This strain does not produce epsilon toxin

Handling information

Handling procedure
  1. Open vial according to enclosed instructions or visit for instructions.
  2. Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #2107 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
  3. Use several drops of the primary broth tube to inoculate a Brucella Blood plate and/or #260 agar slant.
  4. Incubate in an anaerobic atmosphere at 37°C for 24 to 48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
  • Loose screw caps on test tubes in an anaerobic chamber
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained
Handling notes
The presence of cpa and pfoA genes was confirmed by PCR.
Purified genomic DNA of this strain is available as ATCC® 13124D-5™.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Clostridium perfringens (Veillon and Zuber) Hauduroy et al.
Chain of custody
ATCC <-- NCTC <-- Wellcome Lab. CN 1491
Cross references
GenBank AX109537 Sequence 270 from Patent WO0123604.
GenBank AX110098 Sequence 831 from Patent WO0123604.
GenBank M59103 Clostridium perfringens 16S ribosomal RNA.
GenBank J02880 C.perfringens pyruvoyl-dependent histidine decarboxylase (hdc), gene, complete cds.
GenBank S51964 {16S/23S ribosomal DNA spacer region} [Clostridium perfringens, ATCC 13124, Genomic, 239 nt]
GenBank CP00246 Clostridium perfringens ATCC 13124, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

J. Appl. Bacteriol. 22: 1, 1959.

Chalkley L, Van der Westhuyzen I. Penicillin-binding proteins of Clostridia. Curr. Microbiol. 26: 109-112, 1993.

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (tests for specified micro-organisms). Nutritive and selective properties of the media and validity of the test for specified micro-organisms. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.13, 1997

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

View All Curated Citations for this Product

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