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D16 (ATCC® CRL-3281)

Organism: Mus musculus, mouse  /  Cell Type: fat cell  /  Tissue: Inguinal subcutaneous fat depot  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue Inguinal subcutaneous fat depot
Cell Type fat cell
Product Format frozen 1.0 mL
Morphology fibroblast-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain 129SVE
Applications Assay cell model adipogenesis; obesity study; immortalized mouse pre-adipocytes; brown fat; genetic model relevant to brown adipose tissue physiology; can differentiate into brown adipocyte and retain the most relevant aspects of brown adipose tissues.

Brown fat generates heat via the mitochondrial uncoupling protein UCP1, defending against hypothermia and obesity. The isolation of ‘beige’ cells from murine white fat depots which resemble white fat cells show them to have extremely low basal expression of UCP1, but, like classical brown fat, they respond to cyclic AMP stimulation with high UCP1 expression and respiration rates and also beige cells have a gene expression pattern distinct from either white or brown fat and are preferentially sensitive to the polypeptide hormone irisin were produced with different clones. The CRL-3281 is one of the beige clones that, showing evidence that previously identified brown fat deposits in adult humans are composed of beige adipocytes, offers a foundation for studying this mammalian cell type with therapeutic potential for the epidemic of obesity and diabetes.


Storage Conditions vapor phase liquid nitrogen
Images Cell Micrograph of D16 Mouse Cells
Derivation

Primary stromal vascular fraction from inguinal depots of 7-9 week old 129SVE mice (Taconic) was fractioned and cultured as described (J Clin Invest 121(1):96-105, 2011.) Primary SVF cells were immortalized with 3T3 protocol as described (J Cell Biol 17:299-313, 1963.). Limiting dilution of cells into 96-well plates was used to derive subclones of parental immortalized SVF.

Complete Growth Medium 500 mL DMEM/F12 (ATCC® 30-2006)
88 mL FBS (ATCC® 30-2020)
5.9 mL L-Alanyl-L-Glutamine (ATCC® 30-2115)

Seeding Density Between 8.0 x 103 and 2.0 x 104 viable cells/cm2
Subculturing Maintain cells at 40-80% confluency. Split one to three every three to four days. Reaching confluence (>90%) during passaging may reduce the adipogenic potential in the cells and result in poor differentiation later.
Cryopreservation 90% FBS and 10% DMSO
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Volume 1.0 mL
Name of Depositor B Spiegelman
Year of Origin 2010
References

Wu J, et al. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell 150: 366-376, 2012. PubMed: 22796012

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Wu J, et al. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell 150: 366-376, 2012. PubMed: 22796012