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NCTC clone 3526 [derivative of LLC-MK2] (ATCC® CCL-7.2)

Organism: Macaca mulatta, monkey, rhesus  /  Tissue: kidney  /  Disease: normal

Permits and Restrictions

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Organism Macaca mulatta, monkey, rhesus
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age adult
Applications
The cells are positive for keratin by immunoperoxidase staining.
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
The cells are positive for keratin by immunoperoxidase staining.
keratin,The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
keratin
Comments
Grows in chemically defined medium.
The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium NCTC 135 medium, 100%
Subculturing

Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask. Aspirate and dispense into new flasks. The initial subculture interval after the cells are thawed may be somewhat extended (18 to 21 days). Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 3 times per week

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions Temperature: 37°C
Atmosphere: 5% Carbon dioxide (CO2)
Name of Depositor VJ Evans
Deposited As Macaca mulatta
References

Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231

J. Natl. Cancer Inst. 29: 749, 1962.

Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902

. . Am. J. Hyg. 70: 275, 1959.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231

J. Natl. Cancer Inst. 29: 749, 1962.

Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902

. . Am. J. Hyg. 70: 275, 1959.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.