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Primary CD19+ B Cells

PCS-800-018

Product category
Human cells
Product type
Primary cell
Organism
Homo sapiens, human
Morphology
spherical
Tissue
Blood
Disease
Normal
Applications
3D cell culture
Immunology
Toxicology
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Documentation

Biosafety Icon Biosafety Level 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

All tissues used for isolation are obtained under informed consent and conform to HIPAA regulations to protect the privacy of the donor’s Personally Identifiable Information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry Human immunodeficiency virus (HIV) and other bloodborne pathogens. With infectious virus assays or viral antigen assays, even a negative test result may not exclude the possibility of the existence of a latent viral genome or infectious viral particles below the lower limit of detection of that assay.

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Handle as a potentially biohazardous material using universal precautions. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Research purposes, compound screening, immune function assays

Characteristics

Cells per vial
≥ 2.5 x 106
Volume
1.0 mL
Growth properties
Suspension
Age
lot-specific
Ethnicity
Lot-specific
Gender
Lot-specific
Comments
CD19+ B Cells play an essential role in the adaptive immune response. CD19+ B Cells are responsible for the recognition and clearance of antigens in the system and, in the process, creating cells with the purpose of immunosurveillance. The wide range and sensitivity of the B cell to antigens lies in the process in which its receptor, the B cell receptor, develops.

CD19+ B Cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

CD19+ B Cells are tested by flow cytometry for the following surface markers:  

  • CD20+: ≥ 90% 
  • CD45+: ≥ 90%

Note: CD20 is used as a positive identifying marker due to CD19 being saturated during the isolation process.

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.
  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
CD19+ B Cells have a limited lifespan in culture and should be thawed immediately and only prior to their intended use in application specific media. ATCC recommends thawing them in RPMI-1640 (ATCC 30-2001). ATCC does not recommend maintaining CD19+ B Cells in culture in the absence of application-specific growth factors.
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
Refer to the batch specific information for the total number of viable cells recovered from this lot of ATCC® PCS-800-018.
  1. Rapidly thaw the vial in a 37°C water bath.
  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by spraying with 70% ethanol. All operations from this point onward should be carried out under strict aseptic conditions.
  3. Carefully transfer the contents of the vial to a conical tube containing 5mL HBSS (without Ca2+ or Mg2+) containing 10% Human AB serum or 10% Human Serum Albumin (HSA).
  4. Rinse the vial with additional HBSS and transfer to the conical tube.
  5. Bring the volume in the cryovial up to 10 mL with HBSS containing 10% HSA.
  6. Take an aliquot for counting.
  7. Centrifuge the cell suspension at 300xg for 5 minutes at room temperature.
  8. Carefully aspirate the supernatant without disturbing the pellet.
  9. Gently re-suspend the pellet in an appropriate buffer/medium such as RPMI-1640 containing 10% HSA.

Quality control specifications

Virus testing
Human Immunodeficiency virus (HIV): Not detected
Hepatitis C virus (HCV): Not detected
Hepatitis B virus (HBV): Not detected

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Frequently Asked Questions

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Telephone

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Outside the US

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