Primary Bronchial/Tracheal Epithelial Cells; Normal, Human
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Cipollina C, Di Vincenzo S, Gerbino S, et al. Dual anti-oxidant and anti-inflammatory actions of the electrophilic cyclooxygenase-2-derived 17-oxo-DHA in lipopolysaccharide-and cigarette smoke-induced inflammation. Biochim Biophys Acta 1840(7):2299-309, 2014. PubMed: 24594225
Pace E, Di Sano C, Sciarrino S, et al. Cigarette smoke alters IL-33 expression and release in airway epithelial cells. Biochim Biophys Acta 1842(9):1630-7, 2014. PubMed: 24931101
Halim NHA, et al. Isolation and Characterization of Cancer Stem Cells of the Non-Small-Cell Lung Cancer (A549) Cell Line. Methods Mol Biol 1516:371-388, 2016. PubMed: 27032945
Mantalbano AM, et al. Autocrine Acetylcholine, Induced by IL-17A via NFkB and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells. Mediators Inflamm 2016:9063842, 2016. PubMed: 27298519
Pace E, et al. Effects of ceftaroline on the innate immune and on the inflammatory responses of bronchial epithelial cells exposed to cigarette smoke. Toxicol Lett 258:216-226, 2016. PubMed: 27397760