UACC-1598
CRL-3128 ™
- Product category
-
Human cells
- Organism
-
Homo sapiens, human
- Morphology
- epithelial-like
- Tissue
- Ovary
- Disease
-
cystadenocarcinoma; Grade IV
- Applications
-
3D cell culture
- Product format
- Frozen
- Storage conditions
-
Vapor phase of liquid nitrogen
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Specific applications
-
Pathology confirmed that the specimen was a grade IV poorly differentiated papillary serous cystadenocarcinoma.The human ovarian cancer cell line, UACC-1598, was derived from the right ovary of an untreated 78-year-old female.The UACC-1598 cell line expresses the N-myc oncogene, which is rarely amplified in ovarian cancers.UACC-1598 cells express low levels of Epidermal Growth Factor Receptor erbB2, and are negative for ras by ELISA assay.A high copy number amplification of eukaryotic translation initiation factor 5A2 (eIF-5A2) oncogene was detected in this region.
Characteristics
- Growth properties
- Adherent
- Derivation
-
The human ovarian cancer cell line, UACC-1598, was derived from the right ovary of an untreated 78-year-old female. Pathology confirmed that the specimen was a grade IV poorly differentiated papillary serous cystadenocarcinoma.The UACC-1598 cell line contains high-level amplification at 3q26 in the form of double minute chromosomes. A high copy number amplification of eukaryotic translation initiation factor 5A2 (eIF-5A2) oncogene was detected in this region. Overexpression the eIF-5A2 oncogene may play an important role in ovarian cancer pathogenesis.
- Age
- 78 years
- Gender
- Female
- Oncogene
- N-myc, positive; eukaryotic translation initiation factor 5A2 (eIF-5A2), positive; c-erbB2 (low expression); ras, negative
- Genes expressed
- cytokeratin (MAK-6) not expressed; progesterone not expressed; N-myc positive; eukaryotic translation initiation factor 5A2 (eIF-5A2) positive; c-erbB2, low expression; ras negative
- Expression markers
- Epidermal growth factor receptor (EGFR), low expression
- Comments
-
The human ovarian cancer cell line, UACC-1598, was derived from the right ovary of an untreated 78-year-old female. Pathology confirmed that the specimen was a grade IV poorly differentiated papillary serous cystadenocarcinoma.The UACC-1598 cell line expresses the N-myc oncogene, which is rarely amplified in ovarian cancers.UACC-1598 cells express low levels of Epidermal Growth Factor Receptor erbB2, and are negative for ras by ELISA assay.The UACC-1598 cell line contains high-level amplification at 3q26 in the form of double minute chromosomes. A high copy number amplification of eukaryotic translation initiation factor 5A2 (eIF-5A2) oncogene was detected in this region. Overexpression the eIF-5A2 oncogene may play an important role in ovarian cancer pathogenesis.
Handling information
- Unpacking and storage instructions
-
- Check all containers for leakage or breakage.
- Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
- Complete medium
-
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 5%
- 0.01 mg/ml transferrin (final conc.)
- 0.01 mg/ml insulin (final conc.)
- 5 µg/mL (55 U/ml) catalase (final conc.)
- 3.6 µg/mL (0.01 mM) hydrocortisone (final conc.)
- 70 µg/mL (0.5mM) o-phosphoethanolamine (final conc.)
- 10 ng/ml human recombinant epidermal growth factor (EGF) (final conc.)
- 3 ng/ml (0.01 µM) estradiol (final conc.)
- 0.8 ng/ml (1 pM) Na-L-thyroxine (final conc.)
- extra 2 mM glutamine
Note: Do not filter complete medium. - Temperature
-
37°C
- Atmosphere
-
100% Air
- Handling procedure
-
Handling Procedure for Frozen Cells
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
SAFETY PRECAUTION: ATCC highly recommends that protective gloves and clothing always be used and a full face mask always be worn when handling frozen vials. It is important to note that some vials leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vessel exploding or blowing off its cap with dangerous force creating flying debris.
- Subculturing procedure
-
Protocol:
Subculture when cells reach 80% to 90% confluence. These cells pile up and slough off the flask surface if they become over-confluent.
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.- Remove and discard culture medium.
- Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37.0°C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant.
- Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37.0°C in atmospheric air without additional CO2.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended.Medium renewal: every 5 to 7 daysNote: These cells grow very slowly. - Reagents for cryopreservation
- Complete growth medium supplemented with 10% (v/v) fetal bovine serum and 10% DMSO (ATCC 4-X)
Quality control specifications
- Mycoplasma contamination
- Not detected
- STR profiling
- Amelogenin: X CSF1PO: 10 D13S317: 13 D16S539: 11,12 D5S818: 13 D7S820: 7,9 TH01: 7,9.3 TPOX: 8,11 vWA: 14 D3S1358: 16 D21S11: 29,31 D18S51: 18 Penta_E: 7,14 Penta_D: 13,14 D8S1179: 10,11 FGA: 22 D19S433: 13,14 D2S1338: 17,23
History
- Depositors
- K Brown
- Year of origin
- 1989
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Heiskanen MA, et al. Detection of gene amplification by genomic hybridization to cDNA microarrays. Cancer Res. 60(4): 799-802, 2000. PubMed: 10706083
Guan XY, et al. Oncogenic role of eIF-5A2 in the development of ovarian cancer. Cancer Research 64 (12): 4197-4200, 2004. PubMed: 15205331
Clement P, et al. Identification and characterization of eukaryotic initiation factor 5A-2. Eur. J. Biochem. 147(21): 4254-4263, 2003. PubMed: 14622290
Clement P, et al. Differential expression of eIF5A-1 and eIF5A-2 in human cancer cells. FEBS J. 273(6): 1102-1114: 2006. PubMed: 16519677
Guan XY, et al. Isolation of a novel candidate oncogene within a frequently amplified region at 3q26 in ovarian cancer. Cancer Res. 61(9): 3806-3809, 2001. PubMed: 11325856
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