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Cells contain Epstein-Barr virus (EBV) DNA sequences
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Exposure to IL-4 augmented the concentrations of CD23, CD54, and CD58 but diminished the expression of CD21, CD22, and CD38. Incubation with IL-4 for 6 to 8 days led to increased expression of CD11a, CD39, CD40, and to disappearance of CD21 and CD38.
They do not express the terminal deoxynucleotydyl transferase gene (TdT), nor the recombination activating genes RAG-1 and RAG-2, known as markers of the pre-B cell stage. These results show that Farage represents a mature B-cell rather than a pre-B cell.
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Baruch M, et al. Molecular characterization of an unusual non-Hodgkin's B-lymphoma cell line ("Farage") lacking the ability to produce immunoglobulin polypeptide chains. Leuk. Lymphoma 21: 485-495, 1996. PubMed: 9172815
Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
Shubinsky G, et al. The effect of IL-4 on the phenotype of a human B-cell lymphoma line (Farage) lacking immunoglobulin expression. Immunol. Lett. 36: 37-42, 1993. PubMed: 8344715
Shubinsky G, et al. Pathways controlling the expression of surface CD21 (CR2) and CD23 (Fc(epsilon)IIR) proteins in human malignant B cells. Leuk. Lymphoma 25: 521-530, 1997. PubMed: 9250823
Shubinsky G, Schlesinger M. Kinetics of the pleiotropic effect of interleukin 4 on the surface properties of human B-lymphoma cells. Leuk. Lymphoma 15: 333-340, 1994. PubMed: 7866283