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DT40

CRL-2111

DT40 are lymphoblasts that were isolated from the bursa of a patient with lymphoma. It has applications in immunology.
Product category
Animal cells
Organism
Gallus gallus, chicken
Cell type
lymphoblast
Morphology
lymphoblast
Tissue
Bursa
Disease
Lymphoma
Applications
3D cell culture
Immunology
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications

Transfection Host:  These cells may be used for stable transfection studies.

Characteristics

Growth properties
Suspension
Derivation
DT40 is an avian leukosis virus (ALV) induced bursal lymphoma cell line derived from a Hyline SC chicken.
The original lymphoma was induced by viral infection of a 1 day old chicken with Rous associated virus 1 (RAV-1).
Cell suspensions prepared from tumors that developed within the bursa of Fabricus were transferred intravenously into young syngeneic recipient chickens.
After one transfer in vivo, the DT40 cell line was established.
Age
1 day
Tumorigenic
Yes;
Yes, in 2 day old syngeneic chickens; tumors were observed in the spleens, kidneys and livers of recipient animals
Oncogene
C-myc+
Genes expressed
immunoglobulin, IgM (surface)
Comments
The cell line contains proviral DNA sequences integrated upstream from the c-myc proto-oncogene and expresses increased levels of c-myc RNA.

It lacks a normal c-myc gene, but contains two copies of an ALV deregulated myc gene.

The cells retain the ability to rearrange the immunoglobulin light chain gene (IgL).

At the IgL locus, DT40 contains one rearranged and one germline allele.

The cell line exhibits a lymphoblastoid phenotype.

Infectivity assays revealed that DT40 releases low levels of infectious RAV-1.

Both the c-rel gene and the v-rel oncogenes induce major histocompatibility (MHC) class II antigen expression on the DT40 cell line. 

The expression of MHC class II is induced more rapidly by v-rel than c-rel and several weeks after infection, rel induced class II antigen as much as 50 fold more efficiently than c-rel. 

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.
  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine modified to contain 4.5 g/L glucose, 1.5 g/L sodium bicarbonate and 0.05 mM 2-mercaptoethanol, 75%; tryptose phosphate broth, 10%; fetal bovine serum, 10%; chicken serum, 5%
Temperature
37°C
Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

  1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).
  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
  3. It is recommended that the cryoprotective agent be removed immediately.  Centrifuge the cell suspension at approximately 125 xg for 5 to 10 minutes.  Discard the supernatant and resuspend the cell pellet in an appropriate amount of fresh growth medium. 
  4. Transfer the cell pellet to an appropriate size vessel.  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
  5. Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.

   

Subculturing procedure
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 105 cells/mL and maintain between 2 x 105 and 2 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Reagents for cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)

Quality control specifications

Mycoplasma contamination
Not detected

History

Deposited as
Gallus gallus
Depositors
EH Humphries

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Hrdlickova R, et al. v-rel induces expression of three avian immunoregulatory surface receptors more efficiently than c-rel. J. Virol. 68: 308-319, 1994. PubMed: 8254742

Humphries EH, Zhang G. V-rel and C-rel modulate the expression of both bursal and non-bursal antigens on avian B-cell lymphomas. Curr. Top. Microbiol. Immunol. 182: 475-483, 1992. PubMed: 1490388

Baba TW, et al. Cell lines derived from avian lymphomas exhibit two distinct phenotypes. Virology 144: 139-151, 1985. PubMed: 2998040

Buerstedde JM, et al. Light chain gene conversion continues at high rate in an ALV-induced cell line. EMBO J. 9: 921-927, 1990. PubMed: 2155784

Kim S, et al. Ongoing diversification of the rearranged immunoglobulin light-chain gene in a bursal lymphoma cell line. Mol. Cell. Biol. 10: 3224-3231, 1990. PubMed: 2111450

View All Curated Citations for this Product

Frequently Asked Questions

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