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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Transfection Host: These cells may be used for stable transfection studies.
It lacks a normal c-myc gene, but contains two copies of an ALV deregulated myc gene.
The cells retain the ability to rearrange the immunoglobulin light chain gene (IgL).
At the IgL locus, DT40 contains one rearranged and one germline allele.
The cell line exhibits a lymphoblastoid phenotype.
Infectivity assays revealed that DT40 releases low levels of infectious RAV-1.
Both the c-rel gene and the v-rel oncogenes induce major histocompatibility (MHC) class II antigen expression on the DT40 cell line.
The expression of MHC class II is induced more rapidly by v-rel than c-rel and several weeks after infection, rel induced class II antigen as much as 50 fold more efficiently than c-rel.
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
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Hrdlickova R, et al. v-rel induces expression of three avian immunoregulatory surface receptors more efficiently than c-rel. J. Virol. 68: 308-319, 1994. PubMed: 8254742
Humphries EH, Zhang G. V-rel and C-rel modulate the expression of both bursal and non-bursal antigens on avian B-cell lymphomas. Curr. Top. Microbiol. Immunol. 182: 475-483, 1992. PubMed: 1490388
Baba TW, et al. Cell lines derived from avian lymphomas exhibit two distinct phenotypes. Virology 144: 139-151, 1985. PubMed: 2998040
Buerstedde JM, et al. Light chain gene conversion continues at high rate in an ALV-induced cell line. EMBO J. 9: 921-927, 1990. PubMed: 2155784
Kim S, et al. Ongoing diversification of the rearranged immunoglobulin light-chain gene in a bursal lymphoma cell line. Mol. Cell. Biol. 10: 3224-3231, 1990. PubMed: 2111450