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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
Cells contain herpesvirus
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
The consistency of the line's characteristics has made it a useful tool in a wide variety of biochemical, immunological, virological and cell biological studies such as being used as a target cell for EBV superinfection studies. It also has proven useful in chemical induction studies of the latent EBV genome and in the propagation of various (retroviruses) oncornaviruses.
Each cell contains an average of 60 EBV genome copies.
The cells are EBNA positive (EBNA+) and surface immunoglobulin negative (sIg-).
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Durr FE, et al. Studies on the infectivity and cytopathology of Epstein-Barr virus in human lymphoblastoid cells. Int. J. Cancer 6: 436-449, 1970. PubMed: 4321017
Mayyasi SA, et al. The coating reaction of the herpes-type virus isolated from malignant tissues with an antibody present in sera. Cancer Res. 27: 2020-2024, 1967. PubMed: 6073499
The NC-37 cell line was reportedly initiated by W. Korol from peripheral blood from a donor whose serum was positive for EBV antibodies as determined by immunofluorescence and virus coating test.
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.