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1.800.638.6597

293 c18 (ATCC^® CRL-10852^™)

Organism: Homo sapiens, human / Cell Type: transformed with adenovirus 5 DNA / Tissue: kidney /

293 c18 ATCC^® CRL-10852^™

frozen

For-Profit: $632.00 Non-Profit: $537.20

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Permits and Restrictions	View Permits
Organism	Homo sapiens, human
Tissue	kidney
Cell Type	transformed with adenovirus 5 DNA
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	2 CELLS CONTAIN ADENOVIRUS Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	fetus
Storage Conditions	liquid nitrogen vapor temperature

Derivation	This line is a derivative of 293 (see ATCC CRL-1573) the cells express EBNA1.
Comments	Very high frequencies of transformation are obtained with vectors containing the Epstein-Barr virus (EBV) oriP.

Complete Growth Medium	Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.25 mg/ml G-418, 90%; fetal bovine serum, 10%
Subculturing	Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Twice per week
Cryopreservation	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature
Culture Conditions	Temperature: 37°C

STR Profile	Amelogenin: X CSF1PO: 11,12 D13S317: 12,14 D16S539: 9 D5S818: 8,9 D7S820: 11,12 TH01: 7,9.3 TPOX: 11 vWA: 16,19

Name of Depositor	Stanford University
Deposited As	Homo sapiens
References	Murphy AJM, et al. cDNA expression cloning in human cells using the pλDR2 episomal vector system. Methods 4(2):111–131,1992.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
References	Murphy AJM, et al. cDNA expression cloning in human cells using the pλDR2 episomal vector system. Methods 4(2):111–131,1992.