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293 c18 (ATCC® CRL-10852)

Organism: Homo sapiens, human  /  Cell Type: transformed with adenovirus 5 DNA  /  Tissue: kidney  / 

Permits and Restrictions

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Organism Homo sapiens, human
Cell Type transformed with adenovirus 5 DNA
Product Format frozen
Morphology epithelial
Culture Properties adherent

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age fetus
Storage Conditions liquid nitrogen vapor temperature
This line is a derivative of 293 (see ATCC CRL-1573) the cells express EBNA1.
Very high frequencies of transformation are obtained with vectors containing the Epstein-Barr virus (EBV) oriP.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.25 mg/ml G-418, 90%; fetal bovine serum, 10%
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 12,14
D16S539: 9
D5S818: 8,9
D7S820: 11,12
TH01: 7,9.3
TPOX: 11
vWA: 16,19
Name of Depositor Stanford University
Deposited As Homo sapiens

Murphy AJM, et al. cDNA expression cloning in human cells using the pλDR2 episomal vector system. Methods 4(2):111–131,1992.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation

Murphy AJM, et al. cDNA expression cloning in human cells using the pλDR2 episomal vector system. Methods 4(2):111–131,1992.