To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at sales@atcc.org. For Technical questions please contact tech@atcc.org. Thank you.
X

HL-60 purified DNA, [3 µg] (ATCC® CCL-240D-2)

Organism: Homo sapiens, human  /  Focus: Oncology  / 

Permits and Restrictions

View Permits

Focus Area Oncology
Product Format frozen 3 µg, target concentration 100 ng/µL Genomic DNA stored in 1X TE buffer
Storage Conditions -20°C or colder
Comments

DNA taken from HL-60 (ATCC® CCL-240™) Cell Line.

Cell Line Description:

The HL-60 cell line is a human acute myeloid leukemia cell line.

The HL-60 cell line is included in the genetic alteration cell panel MYC (ATCC® TCP-1035).

Spin down before use

Verified mutation in parental cell line: MYC amplification

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications

DNA Quality Control Information*:

Test

Specification

Electrophoresis 

DNA - M.W.

≥48 kb (or higher than uppermost band of the high MW DNA ladder)

Electrophoresis 

RNA - Content

No visible RNA detected in the agarose gel

STR

Identical STR profile to cell line source

DNA Concentration

(PicoGreen® method)

Report results

Purity (A260/A280)

Ratio 1.7 to 2.1

Total DNA amount 

(PicoGreen® method)

≥ 3 µg

Electrophoresis

DNA - Digestion

Verified by restriction enzyme digestion

*Please consult the Certificate of Analysis for lot-specific test results.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation