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iPSC-derived Monocytes; DYS0100

ACS-7030

Human iPSC-derived monocytes
Product category
Human cells
Product type
Primary cell
Organism
Homo sapiens, human
Morphology
rounded
Disease
Normal
Applications
High-throughput screening
Immunology
Stem cell research
Toxicology
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Price: $1,236.00 ea
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Documentation

Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

RPMI-1640 Medium

30-2001

Price: $22.00 ea

Fetal Bovine Serum (FBS)

30-2020

Price: $645.00 ea

Detailed product information

General

Specific applications
Biopharma: Cancer immunology, drug development, and toxicity screening

Characteristics

Cells per vial
Approximately 2.5 x 106
Volume
1.0 mL
Growth properties
Suspension
Age
Newborn
Ethnicity
Unknown
Gender
Male
Comments

Human iPSC-derived Monocytes enables researchers to carry out application which are done using primary CD14+ monocytes. These cells can be used for differentiating into functionally active macrophages and dendritic cells. Additionally these cells can be used for MAT assay, drug development, toxicity screening, and cancer immunology experiments. There is an availability of large number of cell vials from a single donor.

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.
  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
iPSC-derived Monocytes have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining iPSC-derived Monocytes cells in culture in the absence of application-specific growth factors.
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
Refer to the batch specific information for the total number of viable cells.
  1. Using the total number of viable cells, customers have to decide seeding for their experiments and applications (see Spiller, 2015).
  2. Prepare the desired combinations of culture dishes with application specific media (see Spiller, 2015). Place dishes in a 37°C, 5% CO2, humidified incubator and allow the media to pre-equilibrate to temperature and pH for 30 minutes prior to adding cells.
  3. While the culture dishes equilibrate, remove one vial of iPSC-derived Monocytes (ATCC® ACS-7030) from storage and thaw the cells in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1 to 2 minutes).
  4. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point onward should be carried out under strict aseptic conditions.
  5. Add 4ml of RPMI 1640 (ATCC 30-2001) with or without 10% FBS (ATCC 30-2020) or complete growth media for specific applications – into a sterile conical tube. Using a sterile pipette, transfer cells from the cryovial to the conical tube. Centrifuge at 200-300 x g for 5 min, remove supernatant and re suspend the pellet in required medium and take an aliquot for cell counting using Vi-cell
  6. Transfer cell suspension to each of the pre-equilibrated culture dishes in the required seeding density depending the application and gently rock each dishes to evenly distribute the cells.
  7. Place the seeded culture flasks in the incubator at 37°C with a 5% CO2 atmosphere. Incubate for at least 24 hours before processing the cells further.
Cryopreservation
N/A: As this cell line is intended to be consumable no sub culturing and no cryopreservation is recommended.

Quality control specifications

Bacterial and fungal testing
Not detected
Mycoplasma contamination
Not detected
Virus testing
Hepatitis B virus (HBV): Not detected
Hepatitis C virus (HCV): Not detected
Human immunodeficiency virus 1 (HIV-1): Not detected
Human immunodeficiency virus 2 (HIV-2): Not detected
Functional tests
Phagocytosis assay; macrophage and dendritic cell differentiation
STR profiling
Amelogenin: X,Y
CSF1PO: 7,11
D13S17: 11,13
D16S539: 12
D5S818: 11
D7S820: 9,10
TPOX: 8,11
TH01: 9.3
vWA: 14,19
Viability
≥ 80%

History

Depositors
ATCC
Year of origin
2018

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

This material is subject to the following restrictions in addition to those outlined in the ATCC Material Transfer Agreement:

  1. iPS Label License 
  2. This product was generated using Sendai virus vectors from ID Pharma

For information on obtaining additional rights, please contact:

ATCC Licensing
Email: [email protected]

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Panicker LM, et al. Gaucher iPSC derived macrophages produce elevated levels of inflammatory mediators and serve as a new platform for therapeutic development. Stem Cells 32(9): 2338-49, 2014. PubMed: 24801745

Spiller KL, et al. Differential gene expression in human, murine and cell line-derived macrophages upon polarization. Exp Cell Res 347(1): 1-13, 2015. PubMed: 26500109

Frequently Asked Questions