Sphingomonas suberifaciens (van Bruggen et al.) Yabuuchi et al.
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3. Transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate broth tubes at 28oC with shaking for 4 days. Incubate solid medium at 28oC for the same period.
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van Bruggen AH, et al. Rhizomonas suberifaciens gen. nov., sp. nov., the causal agent of corky root of lettuce. Int. J. Syst. Bacteriol. 40: 175-188, 1990.
van Bruggen AH, et al. Corky root of lettuce caused by strains of a gram-negative bacterium from muck soils of Florida, New York, and Wisconsin. Appl. Environ. Microbiol. 55: 2635-2640, 1989.
Validation list no. 70. Int. J. Syst. Bacteriol. 49: 935-936, 1999.
Yabuuchi E, et al. Proposal of Sphingomonas suberifaciens (van Bruggen, Jochimsen and Brown 1990) comb. nov., sphingomonas natatoria (Sly 1985) comb. nov., Sphingomonas ursincola (Yurkov et al. 1997) comb. nov., and emendation of the genus Sphingomonas. Microbiol. Immunol. 43: 339-349, 1999. PubMed: 10385200