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Modified PYNFH Medium [ATCC medium 1034]

327-X

This medium is the recommended formulation for the axenic cultivation of small, free-living amoebae, including Hartmannella, Naegleria, Willaertia, Vahlkampfia, Capsaspora, Nuclearia; the trypanosomatids Crithidia, Blastocrithidia, Herpetomonas, Leptomonas, Phytomonas; and certain ciliates such as Tetrahymena, Dexiostoma, and Glaucoma. It is provided freeze-dried, 1 vial prepares 55 mL of medium. The medium is ready to use upon rehydration with sterile glass-distilled water.
Product category
Media
Formulation

Peptone, 10.0 g

Yeast Extract, 10.0 g

Yeast Nucleic Acid, 1.0 g

Folic Acid, 15.0 mg

Hemin, 1.0 mg

Double Glass Distilled H2O, 880.0 mL

Fetal bovine serum (heat-inactivated), 100.0 mL

Buffer Solution (see below), 20.0 mL

Buffer Solution:

 KH2PO4, 18.1 g

 Na2HPO4, 25.0 g

 Double Glass Distilled H2O, 1.0 L

Comments
The medium is ready to use upon rehydration with sterile glass-distilled water.
Product format
Freeze-dried
Shipping information
1 vial
Storage conditions
2°C to 8°C
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ATCC determined that a biosafety level is not applicable to this material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to complete your own risk assessment and understand any potential hazards associated with the material per your organization’s policies and procedures and any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
This medium is the recommended formulation for the axenic cultivation of small, free-living amoebae, including Hartmannella, Naegleria, Willaertia, Vahlkampfia, Capsaspora, Nuclearia; the trypanosomatids Crithidia, Blastocrithidia, Herpetomonas, Leptomonas, Phytomonas; and certain ciliates such as Tetrahymena, Dexiostoma, and Glaucoma. It is provided freeze-dried, 1 vial prepares 55 mL of medium. The medium is ready to use upon rehydration with sterile glass-distilled water.

Handling information

Handling procedure
  1. Alcohol the crimped aluminum cap and remove it. 
  2. Alcohol the rubber stopper and allow to dry.
  3. Aseptically remove the rubber stopper and aseptically add 55 mL of double glass distilled sterile water. Swirl to completely dissolve the freeze-dried medium.
  4. Aseptically distribute 5 mL of the rehydrated medium to a sterile 16 x 125 mm screw-capped test tube, or distribute 5-10 mL of the rehydrated medium to a sterile 25 cm2 tissue culture flask.
  5. Screw the cap on tightly and store at 2-8°C until ready to use.  Use within approx. 30 days.
  6. When ready to use, equilibrate the tube at the appropriate growth temperature before inoculation with the protist. 
  7. Follow standard procedure for maintaining culture as specified in the product sheet for that protist.

Quality control specifications

Mycoplasma contamination
Not detected

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

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