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Culture Media

Female ATCC scientist in lab coat, safety glasses, and gloves, looking at media in a flask using an inverted microscope.

ATCC protistology culture media spurs growth

ATCC offers a variety of culture media to provide the optimal growth conditions for protist cultures. These mixtures provide the essential proteins, trace elements, amino acids, and carbohydrates needed to support axenic or xenic growth. 

Each medium is tested by ATCC for microbial growth promotion, and they are provided ready to use with all of the reagents needed to support optimal growth. Explore our protist culture media and resources below to support your research.

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Brown, heart-shaped Giardia parasite with pin tendrils.

Keister's Modified TYI-S-33 Giardia Medium

This medium is the recommended formulation for the axenic cultivation of Giardia and Spironucleus. It is prepared as a mixture of casein digest, yeast extract, bovine bile, salts, Diamond’s Vitamin Solution, and heat-inactivated bovine serum. It is provided freeze-dried, complete with 10% bovine serum.

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Blurry blue and black parasite Entamoeba histolytica.

Entamoeba Medium

This medium is used in the axenic cultivation of Entamoeba, Hypotrichomonas, Mastigamoeba, Pentatrichomonas, Tetratrichomonas, Trichomitus, Trichomonas, and Tritrichomonas. It is prepared as a mixture of neutralized liver digest, yeast extract, glucose, salts, vitamins, and heat-inactivated bovine serum.

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Pink, transparent, drop-shaped Tetrahymena eukaryotes with blurry dark pink spots.

Modified PYNFH Medium

This medium is recommended for the axenic cultivation of small, free-living amoebae, including Hartmannella, Naegleria, Willaertia, Vahlkampfia, Capsaspora, Nuclearia; the trypanosomatids Crithidia, Blastocrithidia, Herpetomonas, Leptomonas, Phytomonas; and certain ciliates such as Tetrahymena, Dexiostoma, and Glaucoma.

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Culture Media

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