Derivation
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This line was carried continuously in culture for 5 years before it was sucesssfully cryopreserved.
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Receptor Expression
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gonadotropin releasing hormone (GnRH)
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Genes Expressed
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luteinizing hormone beta (LH); growth hormone (GH); follicle stimulating hormone beta (FSH); prolactin; adrenal corticotropic hormone (ACTH); thyrotropin beta
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Cellular Products
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luteinizing hormone beta (LH); growth hormone (GH); follicle stimulating hormone beta (FSH); prolactin; adrenal corticotropic hormone (ACTH); thyrotropin beta
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Comments
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The line contains cells producing LH, GH, FSH, prolactin, ACTH and thyrotropin beta (hormone production was tested using cells that were grown in medium containing Nu-serum rather than fetal bovine serum).
GnRH receptors are about 2 fold lower in number than on normal pituitary.
The cells contain numerous electron dense secretory granules, golgi complexes, and extended arrays of rough endoplasmic reticulum as well as endogenous rat C-type retrovirus particles.
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