CD19+ B Cells play an essential role in the adaptive immune response. CD19+ B Cells are responsible for the recognition and clearance of antigens in the system and, in the process, creating cells with the purpose of immunosurveillance. The wide range and sensitivity of the B cell to antigens lies in the process in which its receptor, the B cell receptor, develops.
CD19+ B Cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.
CD19+ B Cells are tested by flow cytometry for the following surface markers:
- CD20+: ≥ 90%
- CD45+: ≥ 90%
Note: CD20 is used as a positive identifying marker due to CD19 being saturated during the isolation process.