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Adipose-Derived Mesenchymal Stem Cells; Normal, Human (ATCC® PCS-500-011)

Organism: Homo sapiens, human  /  Tissue: Adipose  /  Cell Type: Mesenchymal Stem

Permits and Restrictions

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Organism Homo sapiens, human
Tissue Adipose
Cell Type Mesenchymal Stem
Morphology Spindle-shaped, fibroblast-like
Growth Properties Adherent
Biosafety Level 1

[These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens.  

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223.

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at]

Human Material Precaution 

All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Normal
Age Adult
Adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, creation of iPS cell lines
Product Format frozen 1 mL
Storage Conditions -130°C or below
The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency.

Mesenchymal Stem Cell Basal Medium, when supplemented with Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum (ATCC® No. PCS-500-040) provides an ideal cell system to propagate mesenchymal stem cells in low serum (2% FBS) conditions. When maintained under optimal growth conditions, ATCC Normal Human Adipose-Derived Mesenchymal Stem Cells have been shown to be multipotent, capable of differentiating down the adipogenic, osteogenic and chondrogenic lineages.
Complete Growth Medium
  1. Obtain one Mesenchymal Stem Cell Growth Kit– for Adipose and Umbilical-derived MSCs - Low Serum (ATCC® No. PCS-500-040) from the freezer; make sure that the caps of all components are tight. 
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. 
  3. Obtain one bottle of Mesenchymal Stem Cell Basal Medium (485 mL) from cold storage. 
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol. 
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.
  6. Table 1. Mesenchymal Stem Cell Growth Kit– for Adipose and Umbilical-derived MSCs - Low Serum  Components 



    Final Concentration

    MSC Supplement

    10 mL

    2% FBS

    5 ng/mL rh FGF basic

    5 ng/mL rh FGF acidic

    5 ng/mL rh EGF


    6 mL

    2.4 mM


    Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of either of the optional components (GA solution or PSA solution) to be added to the complete growth media is summarized in Table 2.


    Table 2. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)



    Final Concentration

    Gentamicin-Amphotericin B Solution

    0.5 mL

    Gentamicin: 10 µg/mL

    Amphotericin B: 0.25 µg/mL

    Penicillin-Streptomycin-Amphotericin B Solution

    0.5 mL

    Penicillin: 10 Units/mL

    Streptomycin: 10 µg/mL

    Amphotericin B: 25 ng/mL

    Phenol Red

    0.5 mL

    33 µM

  7. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle. 
  8. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for two weeks. 
  1. Passage normal adipose-derived stem cells when the culture has reached approximately 70% to 80% confluence.
  2. Warm both the Trypsin-EDTA for Primary Cells (ATCC® No. PCS-999-003) and the Trypsin Neutralizing Solution (ATCC® No. PCS-999-004) to room temperature prior to dissociation. Warm the complete growth medium to 37°C prior to use with the cells.
  3. For each flask, carefully aspirate the spent media without disturbing the monolayer.
  4. Rinse the cell layer one time with 3 to 5 mL D-PBS (ATCC® No. 30-2200) to remove residual medium.
  5. Add pre-warmed trypsin-EDTA solution (1 to 2 mL for every 25 cm2) to each flask.
  6. Gently rock each flask to ensure complete coverage of the trypsin-EDTA solution over the cells, and then aspirate the excess fluid off of the monolayer.
  7. Observe the cells under the microscope. When the cells pull away from each other and round up (typically within 1 to 3 minutes), remove the flask from the microscope and gently tap it from several sides to promote detachment of the cells from the flask surface.
  8. When the majority of cells appear to have detached, quickly add an equal volume of the Trypsin Neutralizing Solution (ATCC® No. PCS-999-004) to each flask. Gently pipette or swirl the culture to ensure all of the trypsin-EDTA solution has been neutralized.
  9. Transfer the dissociated cells to a sterile centrifuge tube and set aside while processing any remaining cells in the culture flask. 
  10. Add 3 to 5 mL D-PBS (ATCC® No. 30-2200) to the tissue culture flask to collect any additional cells that might have been left behind. 
  11. Transfer the cell/D-PBS suspension to the centrifuge tube containing the trypsin-EDTA-dissociated cells.
  12. Repeat steps 10 and 11 as needed until all cells have been collected from the flask. 
  13. Centrifuge the cells at 150 x g for 3 to 5 minutes. 
  14. Aspirate neutralized dissociation solution from the cell pellet and resuspend the cells in 2 to 8 mL fresh, pre-warmed, complete growth medium.
  15. Count the cells and seed new culture flasks at a density of 5,000 viable cells per cm2.
  16. Place newly seeded flasks in a 37°C, 5% CO2 incubator for at least 24 to 48 hours before processing the cells further. Refer to Maintenance for guidelines on feeding.
Volume 1 mL
Cells per Vial One vial contains a minimum of 1 x 106 viable cells.
Sterility Tests
Bacteria and Yeast: Negative
Mycoplasma: Negative
Viral Testing
Hepatitis B: Negative
Hepatitis C: Negative
HIV-1: Negative
HIV-2: Negative
Viability ≥ 70% when thawed from cryopreservation.
Population Doubling Capacity ≥ 10 in complete growth medium and support differentiation
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation

Li Q, et al. Sulforaphane inhibits mammary adipogenesis by targeting adipose mesenchymal stem cells. Breast Cancer Res Treat 141(2):317-24, 2013. PubMed: 24002734

Tan KY, et al. Serum-free media formulations are cell line-specific and require optimization for microcarrierculture. Cytotherapy 17(8):1152-65, 2015. PubMed: 26139547

Sahin E, et al. Resveratrol reduces IL-6 and VEGF secretion from co-cultured A549 lung cancer cells and adipose-derived mesenchymal stem cells. Tumour Biol 37(6):7573-82, 2016. PubMed: 26687643

Gattu AK, et al. Determination of mesenchymal stem cell fate by pigment epithelium-derived factor (PEDF) resultsin increased adiposity and reduced bone mineral content. FASEB J 27(11):4384-94, 2013. PubMed: 23887690

Xu H, et al. Electrospun ultrafine fibrous wheat glutenin scaffolds with three-dimensionally randomorganization and water stability for soft tissue engineering. J Biotechnol 184:179-86, 2014. PubMed: 24862198