Primary Vaginal Epithelial Cells (ATCC® PCS-480-010)

Organism: Homo sapiens, human  /  Tissue: vagina  /  Cell Type: epithelial

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Organism Homo sapiens, human
Tissue vagina
Cell Type epithelial
Morphology polygonal, cobblestone appearance
Growth Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age batch-specific
Gender female
Ethnicity batch-specific
Applications Cancer studies
Microbiological organism to cell interaction
Toxicity studies
Product Format frozen 1.0 mL
Storage Conditions liquid nitrogen vapor phase
Comments Every day, remove medium and feed 5 mL of supplemented medium. However, when cultures reach 50% (or greater) confluence, remove medium and feed with 5 to 8 mL of supplemented medium daily.
Complete Growth Medium One bottle of Vaginal Epithelial Cell Basal Medium (ATCC PCS-480-030) plus Vaginal Epithelial Cell Growth Kit (ATCC PCS-480-040)
Culture Conditions
Atmosphere: air, 5%; carbon dioxide (CO2), 5%
Temperature: 37°C
Subculturing
  1. Passage normal vaginal epithelial cells when culture has reached approximately 85 to 100% confluence, and are actively proliferating.
  2. Warm both the Trypsin-EDTA for Primary Cells (ATCC PCS-999-003) and the Trypsin Neutralizing Solution (ATCC PCS-999-004) to room temperature prior to dissociation. Warm complete growth medium to 37°C prior to use with the cells.
  3. For each flask, carefully aspirate the spent media without disturbing the monolayer.
  4. Briefly rinse the cell layer with 3 to 5 mL DPBS (ATCC 30-2200) to remove residual traces of serum and then aspirate and discard the DPBS.
  5. Add pre-warmed trypsin-EDTA solution (2 to 3 mL for every 25 cm2) to each flask.
  6. Gently rock each flask to ensure complete coverage of the trypsin-EDTA solution over the cells.
  7. Observe the cells under the microscope. When the cells pull away from each other and round up (typically within 3 to 5 minutes), remove the flask from the microscope and gently tap it from several sides to promote detachment of the cells from the flask surface.
  8. When the majority of cells are detached, quickly add an equal volume of Trypsin Neutralizing Solution (ATCC® PCS-999-004) to each flask. Gently pipette or swirl the culture to ensure all of the trypsin-EDTA solution has been neutralized.
  9. Transfer the dissociated cells to a sterile centrifuge tube and set aside while processing any remaining cells in the flask.
  10. Add 3 to 5 mL Trypsin Neutralizing Solution to the flask to collect any remaining dissociated cells. Transfer remaining cells into the centrifuge tube.
  11. Repeat steps 10 as needed until all cells have been collected from the flask.
  12. Centrifuge the cells at 150 x g for 3 to 5 minutes.
  13. Carefully aspirate the neutralized dissociation solution from the cell pellet and re-suspend the cells in 5 to 8 mL fresh, pre-warmed, complete growth medium.
  14. Count the cells and seed new flasks at a density of 5,000 cells per cm2.
  15. Place freshly seeded flasks in a 37°C, 5% CO2 incubator for at least 24 to 48 hours before processing the cells further. Refer to Maintenance for guidelines on feeding.
Volume 1.0 mL
Cells per Vial ≥ 5.0 x 105
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viral Testing Hepatitis B: None detected
Hepatitis C: None detected
Human immunodeficiency virus 1: None detected
Human immunodeficiency virus 2: None detected
Viability ≥ 70%
Population Doubling Time when seeded at 5,000 cells per cm2, cells reach 95% confluence in 4 to 6 days
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQs
  1. Recommended seeding density - PCS-480-010


    Date Updated: 2/14/2018

  2. HVEC marker expression

    ATCC has tested the HVEC cells by ICC for positive expression of CK8, CK14, CK18, PanCK, AQP2,


    Date Updated: 2/14/2018
  3. Subculturing HVECs - PCS-480-010

    It is highly recommended to passage the  HVECs whey they reach  approximately 85% confluency.


    Date Updated: 2/14/2018
  4. HVEC application - PCS-480-010


    Date Updated: 2/14/2018

  5. Population doubling level - PDL of HVECc - PCS-480-010


    Date Updated: 2/14/2018

References

Sharkey DJ, et al. Seminal plasma differentially regulates inflammatory cytokine gene expression in human cervical and vaginal epithelial cells. Mol Hum Reprod 13(7): 491-501, 2007. PubMed: 17483528

Steele C, Fidel PL. Cytokine and chemokine production by human oral and vaginal epithelial cells in response to Candida albicans. Infect Immun 70(2): 577-583, 2002. PubMed: 11796585

Fichorova RN, et al. Distinct proinflammatory host responses to Neisseria gonorrhoeae infection in immortalized human cervical and vaginal epithelial cells. Infect. Immun. 69: 5840-5880, 2001. PubMed: 11500462