Primary Gingival Keratinocytes (ATCC® PCS-200-014)

Organism: Homo sapiens, human  /  Tissue: Jaw  /  Cell Type: Keratinocyte

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Organism Homo sapiens, human
Tissue Jaw
Cell Type Keratinocyte
Morphology epithelial-like; cobblestone appearance; cells are rounded, not flat; cells display a high mitotic index; at near 80% confluence, the cells will be associated with each other in colonies.
Growth Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 60 years
Gender female
Ethnicity Caucasian
Applications The normal gingival epithelial cells, like the dermal epithelial cells express different types of anti-microbial peptides. Epithelial antimicrobial peptides play an important role in determining the outcome of the host-pathogen interaction at the oral mucosal barrier. Gingival keratinocytes may have important applications in antibiotic treatment, dental implants, and many other applications for oral biology research.
Product Format frozen 1.0 mL
Storage Conditions liquid nitrogen vapor phase
Images ATCC PCS-200-014 KC Markers Images Cell Micrograph of ATCC PCS-200-014
Comments Cryopreserved human oral keratinocytes derived from adult gingival tissue. Oral keratinocytes are cryopreserved in P2: After isolation from tissue the cells are cultured for two passages and then cryopreserved. Vials contain 500,00 cells in one mL cryopreservation solution.
Complete Growth Medium One bottle of Dermal Cell Basal Medium (ATCC® PCS-200-030™) plus one Keratinocyte Growth Kit (ATCC® PCS-200-040™).
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Rinse the cell layer with DPBS solution for 2 minutes to remove all traces of serum that contains trypsin inhibitor.
  3. Add 5.0 to 7.0 mL of Trypsin-EDTA solution to the flask and incubate at 37°C .Observe cells under an inverted microscope until cell layer is dispersed (usually within 4 to 6 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Add 5.0 to 7.0 mL of Trypsin Neutralizing Solution (ATCC® PCS-999-004™) Centrifuge at 125 x g; 10 ± 2 minutes. Discard supernatant and resuspend the cell pellet with 8 mL of complete growth media. Gently break cell pellet by pipetting repeatedly.
  4. Count cells. Seed 2,500 to 5,000 viable cells per cm2. Add appropriate volume of the cell suspension to new culture vessels.
  5. Incubate cultures at 37°C.
Change media every 2-3 days
Subculture when cells reach 75-80% confluence. Seeding density should be 2,500 to 5,000 viable cells per cm2
Volume 1.0 mL
Cells per Vial Approximately 5 x 105 cells
Sterility Tests Bacteria and Yeast: No growth
Mycoplasma: No growth
Viral Testing Hepatitis B: None detected
Hepatitis C: None detected
HIV-1: None detected
HIV-2: None detected
Viability ≥ 70%
Population Doubling Capacity 15 PDL
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Hou C, et al. MircoRNA-509 acts as a tumor suppressor in tongue squamous cell carcinoma by targeting epidermal growth factor receptor. Mol Med Rep 16(5):7245-7252, 2017. PubMed: 28944863