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Purple spheres of Enterococcus faecalis bacteria.

Tips and Techniques for Successfully Growing Bacteria in Culture Webinar

February 17, 2022


Identifying the ideal culture conditions for a bacterial strain can be challenging, particularly for fastidious species that require specialized growth conditions. For nearly a century, ATCC scientists have propagated and maintained numerous microbial species, including difficult-to-grow bacteria such as Mollicutes and extremophiles. In this webinar, we will cover the various methods and techniques used at ATCC to achieve successful growth for a wide variety of bacteria and will detail the troubleshooting tips our scientists use for culturing fastidious strains. Plan to join as we will have a lively Q&A session after the webinar to answer your questions and concerns about bacterial propagation.

Key Points

  • Specific growth requirements should be tailored to ensure the successful propagation of individual bacteria strains. Different atmospheres, temperature ranges and other growth conditions will be discussed.
  • New bacterial isolates may present unique growth issues.
  • A rational approach to troubleshooting can ensure the resolution of poor growth.

Watch The Presentation


Nancy Krueger Senior Biologist, ATCC

Nancy Krueger

Lead Biologist, ATCC

Nancy Krueger is a Lead Biologist in the Manufacturing, Science, and Technology (MSAT) Bacteriology department at ATCC. During the last 11 years, she has developed an in-depth knowledge of technology transfer, quality control, and propagation of a variety of bacteria, fungi, and yeast. Ms. Krueger is also experienced in studies determining the ideal propagation and test methods for anaerobes, optimization of the propagation methods of bacteriophage, and enumeration of Mycoplasma. Prior to working at ATCC, she worked in healthcare in a hospital laboratory and emergency department. Ms. Krueger has a bachelor’s degree in Biology from George Mason University.