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Thermoanaerobacter pseudethanolicus

33223

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Thermoanaerobacter pseudethanolicus strain 39E was isolated in 1978 from a microbial mat at Octopus Spring in Yellowstone National Park. This whole-genome sequenced anaerobic thermophile is known to produce ethanol from starch.
Product category
Bacteria
Product type
Anaerobe
Extremophile
Strain designation
39E
Type strain
No
Genome sequenced strain
Yes
Isolation source
Microbial mat in Octopus Spring at Yellowstone National Park
Geographical isolation
United States; Wyoming
Applications
Bioinformatics
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

Biosafety Icon Biosafety Level 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Degrades starch
Produces ethyl alcohol ethanol
Produces glucoamylase
Produces pullulanase pullulan 6-glucanohydrolase
Produces thermostable pullulanase and glucoamylase
Produces ethanol from starch
Preceptrol
No

Characteristics

Extremophile type
Thermophile
Comments
Taxonomy
Genome sequenced strain

Handling information

Medium
Temperature
65°C
Atmosphere
Anaerobic
Handling procedure
  1. Sterilize the top of the Balch tube by spraying it with 70% ethanol and then flame the top.
  2. If needed exchange the gas in the test tube for 80% N2 -20% CO2.
  3. When the Balch tube is ready to inoculate, open the vial.  With a sterile anaerobic syringe tipped with a 22-gauge needle withdraw 0.5 ml of reduced #1118 medium and add it to the freeze dried pellet.  Immediately place the vial under a gentle stream of oxygen free gas.
  4. Using the same anaerobic syringe draw the rehydrated  culture up and inoculate the same tube of #1118 broth. Plate 0.1 ml of the inoculated culture onto a non-selective medium and incubate aerobically at 37oC.  Use 0.1 ml of the inoculated culture to inoculate a non-selective aerobic broth and an additional tube of #1118 broth.  Incubate the non-selective aerobic broth tubes at 37oC.  Incubate the #1118 broth culture at 65oC.
  5. Growth should be detected in the #1118 broth within 48 to 72 hours.  There should be no growth detected on the aerobic plate or in the aerobic broth.

 

ANAEROBIC CONDITIONS:

  1. Resazurin is a commonly used redox indicator that is pink when the redox potential is above -50 mv.,  and colorless when the redox potential is below -110 mv. i.e. highly reducing.  Most strict anaerobes require this low redox potential for optimum growth.
  2. To obtain a fully reduced medium, it is necessary that the medium be anoxic and that a reducing agent be added.  Common reducing agents are sodium sulfide, cysteine, dithiothreitol, and titanium citrate.
 
Handling notes
Gram negative curved rods of varying length which form chains and spores.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Clostridium thermohydrosulfuricum Klaushofer and Parkkinen
Depositors
JG Zeikus
Type of isolate
Environmental
Year of origin
1978
Special collection
National Park Service (NPS) Special Collection
Cross references
GenBank Y08557 T. ethanolicus melA and lacA genes.
GenBank L09164 Thermoanaerobacter ethanolicus (ATCC 33223) 16S ribosomal RNA (16S rRNA) gene.
GenBank U49975 Thermoanaerobacter ethanolicus secondary-alcohol dehydrogenase (adhB) gene, complete cds.
GenBank E03575 DNA sequence encoding clostridium thermohydrosulfuricum thermo-resistant xylose isomerase.
GenBank CP000924 Thermoanaerobacter pseudethanolicus ATCC 33223, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Material Transfer Agreement Addendum for National Park Service Products

For every order of this item, you must provide a signed Material Transfer Agreement Addendum for National Park Service Products. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item. Email the signed addendum to [email protected] with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Hyun HH, Zeikus JG. General biochemical characterization of thermostable pullulanase and glucoamylase from Clostridium thermohydrosulfuricum. Appl. Environ. Microbiol. 49: 1168-1173, 1985.

Zeikus JG, Hyun HH. Co-culture production of thermostable enzymes and ethanol. US Patent 4,604,352 dated Aug 5 1986

Lee YE, et al. Taxonomic distinction of saccharolytic thermophilic anaerobes: description of Thermoanaerobacterium xylanolyticum gen. nov., sp. nov., and Thermoanaerobacterium saccharolyticum gen. nov., sp. nov.; reclassification of Thermoanaerobium brockii, Clostridium thermosulfurogenes, and Clostridium thermohydrosulfuricum E100-69 as Thermoanaerobacter brockii comb. nov., Thermoanaerobacterium thermosulfurigenes comb. nov., and Thermoanaerobacter thermohydrosulfuricus comb. nov., respectively; and transfer of Clostr. Int. J. Syst. Bacteriol. 43: 41-51, 1993.

algal mat, Yellowstone National Park

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