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Bacillus cereus Frankland and Frankland


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This whole-genome sequenced strain of Bacillus cereus has applications in food testing. This bacterial type strain is known to produce the restriction endonuclease Bce14579I.
Product category
Product type
Preceptrol culture
Strain designation
[BCRC 10603, CCM 2010, CCUG 7414, CIP 66.24, DSM 31, HAMBI 1887, HAMBI 1905, IAM 12605, JCM 2152, LMG 6923, NBRC 15305, NCCB 75008, NCIMB 9373, NCTC 2599, NRRL B-3711, VKM B-504]
Type strain
Genome sequenced strain
Food testing
Food production research
Agricultural research
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Produces restriction endonuclease Bce14579I
Food testing

Potential strain for single cell protein production for food or feed

Potential plant growth promoting strain; Biofertilizer



Genome sequenced strain
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
  5. Incubate the tubes and plate at 30°C for 24 hours.
Handling notes
Purified genomic DNA of this strain is available as ATCC®14579D-5™.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Bacillus cereus Frankland and Frankland
RE Gordon
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Cross references
GenBank AE016877 Bacillus cereus ATCC 14579, complete genome.
GenBank X98626 B.cereus SNF2 gene, partial
GenBank Y10927 B.cereus bct1 and bcglr genes.
GenBank AX110270 Sequence 1003 from Patent WO0123604.
GenBank AX111054 Sequence 1787 from Patent WO0123604.
GenBank AX111073 Sequence 1806 from Patent WO0123604.
GenBank AX111085 Sequence 1818 from Patent WO0123604.
GenBank L48555 Bacillus cereus (library ATCC 14579) vrrA gene.
GenBank L37572 Bacillus cereus GyrB-GyrA intergenic spacer, DNA sequence.
GenBank L37576 Bacillus cereus 16S-23S rRNA intergenic spacer, DNA sequence.
GenBank AJ304815 Bacillus cereus partial clo1 gene for putative cereolysin O.
GenBank AJ304816 Bacillus cereus partial clo2 gene for putative cereolysin O.
GenBank AJ007952 Bacillus cereus celR, bct499b, chrA, ydjC and celF genes, partial.
GenBank AJ272333 Bacillus cereus cheA gene for histidine kinase, strain ATCC 14579.
GenBank AJ007794 Bacillus cereus hblC, hblD, hblA, hblB, kin1 and gerX genes, partial.
GenBank AJ272330 Bacillus cereus fliY gene for component of flagellar switch complex, strain ATCC 14579.
GenBank AJ007795 Bacillus cereus yndD, yndE, yndF, proC, yfnA, orf6, sspC, trrA, and hblC genes, partial.
GenBank AJ243712 Bacillus cereus yvfR (partial), yvfS, yvfT, yvfU, nprB, plcR, ykoW, ywjD and ywjE genes and ORF2.
GenBank AJ237785 Bacillus cereus ATCC 14579 21.2 kb chromosomal locus containing genes encoding enterotoxic hemolysin BL and surrounding regions; yndD (partial), yndE, yndF, proC, yfnA, sspC, trp1, hblC, hblD, hblA, hblB, shk1 and ger (partial) genes.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Ivanova N., et al. Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis. Nature 423: 87-91, 2003. PubMed: 12721630

Leonard C, et al. Integrated physical and genetic mapping of Bacillus cereus and other gram-positive bacteria based on IS231A transposition vectors. Infect. Immun. 66: 2163-2169, 1998. PubMed: 9573103

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Shibata T, et al. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains. J. Bacteriol. 128: 473-476, 1976. PubMed: 824277

View All Curated Citations for this Product

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